Cell Invasion Assay (Collagen IV), 96-well, 8 µm (ab235888)

Overview

  • Product name

    Cell Invasion Assay (Collagen IV), 96-well, 8 µm
    See all Cell Invasion kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Species reactivity

    Reacts with: Mouse, Human
  • Product overview

    Cell Invasion Assay (Collagen IV), 96-well, 8 µm (ab235888) utilizes a Boyden chamber coated with Collagen IV, where the cells invade the matrix and then migrate through a semi-permeable membrane in the Boyden chamber in response to stimulants or inhibitory compounds. The percent cell invasion can be analyzed directly in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.

  • Notes

    Cell invasion is the ability of cells to migrate from one area to another through an extracellular matrix. Cell invasion is exhibited by both normal cells as well as cancerous cells in response to specific external signals, including chemical and mechanical stimuli. During invasion, extracellular matrix is enzymatically degraded by cellular proteases before cells migrate to the new location. Cell invasion is required for normal processes such as wound repair, vasculature formation and the inflammatory response as well as the abnormal invasion of tissues by tumor cells during metastasis.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cell Dissociation Solution   1 x 10ml
    Cell Dye 1 vial
    Cell Invasion Chamber 1 unit
    Collagen IV 1 x 5ml
    Control Invasion Inducer 1 x 300µl
    Wash Buffer   1 x 50ml

Images

  • HT-1080 cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol.

  • NIH-3T3 and HT-1080 cells were starved overnight and treated with Control (Cnt) Invasion Inducer or remain untreated (No Treatment). Treatment with Control Invasion Inducer demonstrated a significant increase in invasion of HT 1080 cells as compared to 3T3-NIH control cells.

Protocols

References

ab235888 has not yet been referenced specifically in any publications.

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