Overview

  • Product name
    Cell Lysis Buffer
  • Tested applications
    Suitable for: WB, IPmore details
  • General notes

    ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

    Recommended for preparing samples for use in western blotting and immunoprecipitation. For use with adherent or suspension cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at -20°C. Stable for 12 months at -20°C
  • Storage buffer
    pH: 7.50
    Constituents: 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate, 8.8% Sodium chloride, 3.15% Tris HCl, 10% Triton-X-100, 0.38% EGTA, 0.001% Leupeptin, 0.19% Sodium orthovanadate, 0.216% Beta glycerophosphate
  • Concentration information loading...
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab152163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Protocols

References

This product has been referenced in:
  • Raikwar SP  et al. Targeted Gene Editing of Glia Maturation Factor in Microglia: a Novel Alzheimer's Disease Therapeutic Target. Mol Neurobiol N/A:N/A (2018). Read more (PubMed: 29704201) »
  • Isaac C  et al. Mycolactone displays anti-inflammatory effects on the nervous system. PLoS Negl Trop Dis 11:e0006058 (2017). Read more (PubMed: 29149212) »
See all 7 Publications for this product

Customer reviews and Q&As

Answer

For ab126615, I have attached the WB image with HepG2 cell lysate.

As for the cell lysis buffer used for the WB with ab108315, it is product ab152163 (https://www.abcam.com/index.html?datasheet=152163) that was used. The buffer composition is given on the datasheet:
pH: 7.50
Constituents: 0.216% Beta glycerophosphate, 0.19% Sodium orthovanadate, 0.001% Leupeptin, 0.38% EGTA, 10% Triton-X-100, 3.15% Tris HCl, 8.8% Sodium chloride, 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate

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