Cell Lysis Buffer (ab152163)
Overview
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Product name
Cell Lysis Buffer -
Tested applications
Suitable for: WB, IPmore details -
General notes
ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.
Recommended for preparing samples for use in western blotting and immunoprecipitation. For use with adherent or suspension cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Form
Liquid -
Storage instructions
Store at -20°C. Stable for 12 months at -20°C -
Storage buffer
pH: 7.50
Constituents: 0.29% Sodium EDTA, 1.12% Sodium pyrophosphate decahydrate, 8.8% Sodium chloride, 3.15% Tris HCl, 10% Triton-X-100, 0.38% EGTA, 0.001% Leupeptin, 0.19% Sodium orthovanadate, 0.216% Beta glycerophosphate -
Concentration information loading... -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab152163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
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WB
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
Datasheets and documents
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SDS download
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Datasheet download
References (21)
ab152163 has been referenced in 21 publications.
- Gonella-Diaza AM et al. Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows. Int J Mol Sci 22:N/A (2021). PubMed: 33477993
- Hsu SH et al. Peptidoglycan mediates Leptospira outer membrane protein Loa22 to toll-like receptor 2 for inflammatory interaction: a novel innate immune recognition. Sci Rep 11:1064 (2021). PubMed: 33441663
- EzEldeen M et al. Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior. Sci Rep 11:5687 (2021). PubMed: 33707502
- Wang X & Bai M CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro. BMC Cardiovasc Disord 21:51 (2021). PubMed: 33526034
- Zhao YH et al. Curcumin prevents renal cell apoptosis in acute kidney injury in a rat model of dry-heat environment heatstroke via inhibition of the mitochondrial apoptotic pathway. Exp Ther Med 21:126 (2021). PubMed: 33376508