Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Product nameCell Migration/Chemotaxis Assay Kit (24-well, 3 µm)
See all Cell Migration/Chemotaxis kits
Sample typeAdherent cells, Suspension cells
Species reactivityReacts with: Human
Cell Migration/Chemotaxis Assay Kit (24-well, 3 µm) (ab235899) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. Our assay is easy to use, sensitive and adaptable to high-throughput systems.
Cell migration is a process by which cells move from one location to another. Cell mobility is observed in unicellular organisms, and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical and mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 12 tests Cell Dissociation Solution 1 x 6ml Cell Dye 1 x 1.5ml Cell Migration Chamber 1 unit Control Migration Inducer 1 x 300µl Wash Buffer 1 x 25ml
Erythroleukemia cells were starved overnight and treated with Control Migration Inducer or left untreated (control cells). Treatment with Control Migration Inducer demonstrated a significant increase in migration as compared to control cells.
Erythroleukemia cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Dye and RFU (Ex/Em = 530/590 nm) was measured.
ab235899 has not yet been referenced specifically in any publications.