Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693)

Overview

  • Product name

    Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm)
    See all Cell Migration/Chemotaxis kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Product overview

    Cell Migration/Chemotaxis Assay Kit (96-well, 5 µm) (ab235693) utilizes a Boyden chamber, where the cells migrate through a semi-permeable membrane under different stimuli. Cell migration can be analyzed directly by reading fluorescence (Ex/Em = 530/590 nm) in a plate reader. The assay is easy to use, sensitive and adaptable to high-throughput systems.

  • Notes

    Cell migration is a process by which cells move from one location to another. Cell motility is observed in unicellular organisms, and is essential for the development and maintenance of multicellular organisms. Cells often migrate in response to specific external stimuli, including chemical & mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cell Dissociation Solution   1 x 10ml
    Cell Dye 1 x 1.5ml
    Cell Migration Chamber  1 unit
    Control Migration Inducer  1 x 300µl
    Wash Buffer   1 x 50ml

Images

  • Monocytes/macrophage cells were starved overnight and treated with Control Migration Inducer for 24 h, 48 h or left untreated (Cnt: control cells). Treatment with Control Migration Inducer demonstrated a significant increase in migration with time as compared to control cells.

  • Monocytes/macrophage cells were harvested, counted and serially diluted to obtain desired cell number. Cells were incubated according to the protocol with Cell Dye and RFU (Ex/Em = 530/590 nm) was measured.

Protocols

References

ab235693 has not yet been referenced specifically in any publications.

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