• Product name
    Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter
    See all Cell viability/proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (quantitative)
  • Species reactivity
    Reacts with: Other species
    Predicted to work with: Mammals
  • Product overview

    Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.

    The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.

    Main features of CytoLabeling Deep Red Reagent:

    • It eliminates the dye efflux drawback associated with CFSE

    • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required

    • It is retained up to 9 generations after staining

    • Fluorescence is preserved upon formaldehyde fixation

    This product can be used for imaging or flow cytometric analysis.

    Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide

  • Notes

    Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

  • Platform
    Flow cytometer



  • Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).

  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1:1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.



This product has been referenced in:
  • Muenzner JK  et al. Generation and characterization of hepatocellular carcinoma cell lines with enhanced cancer stem cell potential. J Cell Mol Med 22:6238-6248 (2018). Read more (PubMed: 30280520) »
See 1 Publication for this product

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