Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter (ab176736)

Overview

  • Product name
    Cell Proliferation Staining Reagent - Deep Red Fluorescence - Cytopainter
    See all Cell viability/proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (quantitative)
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mammals
  • Product overview

    Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.


    The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.


    Main features of CytoLabeling Deep Red Reagent:



    • It eliminates the dye efflux drawback associated with CFSE

    • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required

    • It is retained up to 9 generations after staining

    • Fluorescence is preserved upon formaldehyde fixation


    This product can be used for imaging or flow cytometric analysis.

  • Notes

    Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

  • Tested applications
    Suitable for: Flow Cytmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab176736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

Images

  • Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).

  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1:1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

Protocols

References

ab176736 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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