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Cell Proliferation Staining Reagent - Deep Red Fluorescence | Cytopainter (ab176736) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 628-643 nm excited with a red laser at Ex = 633 nm.
The CytoLabeling Red Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.
Main features of CytoLabeling Deep Red Reagent:
This product can be used for imaging or flow cytometric analysis.
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
|Components||500 tests||1000 tests|
|CytoLabeling Red Reagent||1 vial||1 x 2 vials|
Our Abpromise guarantee covers the use of ab176736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736).
Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Red Fluorescence (ab176736). Jurkat cells (2x106 cells/mL) were stained with ab176736 on Day 0. The cells were passed serially at 1:1 ratio for 11 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.
ab176736 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"