Overview

  • Product name
    Cell Proliferation Staining Reagent - Green Fluorescence - Cytopainter
    See all Cell viability/proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (quantitative)
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Cell Proliferation Staining Reagent - Green Fluorescence | Cytopainter (ab176735) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. The CytoLabeling Green Reagent is designed to label cells at Em = 511-525 nm excited with a blue laser at Ex = 488 nm.


    The CytoLabeling Green Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used.


    Main features of CytoLabeling Green Reagent:



    • It eliminates the dye efflux drawback associated with CFSE

    • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required

    • It is retained up to 9 generations after staining

    • Fluorescence is preserved upon formaldehyde fixation


    This product can be used for imaging or flow cytometric analysis.


    Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide

  • Notes

    Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

  • Platform
    Flow cytometer, Fluorescence microscope

Properties

Images

  • Emission and Excitation spectra for CytoPainter Cell Proliferation Staining Reagent – Green Fluorescence (ab176735).

  • Cell tracking assay with CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735) and CFSE. Jurkat cells (2x106 cells/mL) were stained with ab176735 or CFSE (0.5 µM) on Day 0. The cells were passed serially at 1:1 ratio for 9 days. Fluorescence intensity was measured with a flow cytometer in FL1 channel on the day after passage. Successive generations were represented by different colors.

Protocols

References

ab176735 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Mouse sample in ICC/IF application

Excellent Excellent 5/5 (Ease of Use)
Abreviews
The product is easy to use and works perfect. I use it in primary microglia cells, they are quite delicate but the staining didn't affected the survival of the cells.

Abcam user community

Verified customer

Submitted Jan 19 2018

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