Overview

  • Product name
    Cell Proliferation Staining Reagent - Orange Fluorescence - Cytopainter
    See all Cell viability/proliferation kits
  • Sample type
    Adherent cells, Suspension cells
  • Assay type
    Cell-based (quantitative)
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    Cell Proliferation Staining Reagent - Orange Fluorescence | Cytopainter (ab176737) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 542-556 nm excited with a blue laser at Ex = 488 nm or with a green laser at Ex = 531 nm.


    The CytoLabeling Orange Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.


    Main features of CytoLabeling Orange Reagent:



    • It eliminates the dye efflux drawback associated with CFSE

    • It is compatible with the cell culture medium in the staining cells prior analysis - no washing required

    • It is retained up to 9 generations after staining

    • Fluorescence is preserved upon formaldehyde fixation


    This product can be used for imaging or flow cytometric analysis.


    Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide

  • Notes

    Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.

  • Platform
    Flow cytometer

Properties

Protocols

References

ab176737 has not yet been referenced specifically in any publications.

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