Cell Proliferation Staining Reagent - Orange Fluorescence - Cytopainter (ab176737)
Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Proliferation Staining Reagent - Orange Fluorescence - Cytopainter
See all Cell viability/proliferation kits -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Cell Proliferation Staining Reagent - Orange Fluorescence | Cytopainter (ab176737) is a powerful tool to analyze heterogeneous cell populations where multicolor analysis is required. This reagent is designed to label cells at Em = 542-556 nm excited with a blue laser at Ex = 488 nm or with a green laser at Ex = 531 nm.
The CytoLabeling Orange Reagent dye contained in this kit is functionally similar to CFSE and can be used for the multicolor applications where either GFP or FITC-labeled antibody is used, since the CytoLabeling Reagents staining reagents have either excitation and emission spectra distinct from CFSE and its fluorescein analogs.
Main features of CytoLabeling Orange Reagent:
- It eliminates the dye efflux drawback associated with CFSE
- It is compatible with the cell culture medium in the staining cells prior analysis - no washing required
- It is retained up to 9 generations after staining
- Fluorescence is preserved upon formaldehyde fixation
This product can be used for imaging or flow cytometric analysis.
Review other dyes and kits for cell proliferation staining, or the live cell staining fluorescent dyes guide
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Notes
Uniform labeling of live cells with a dye containing a cell-retaining group to allow retention in proliferating cells. It enables multicolor labeling and functional analysis of live cells in combination with other fluorochromes. The dye is retained for up to 9 generations after staining.
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Platform
Flow cytometer
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 500 tests 1000 tests CytoLabeling Orange Reagent 1 vial 2 vials -
Research areas
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Relevance
Cell proliferation is the multiplication or reproduction of cells, as a result of cell growth and cell division, resulting in the expansion of a cell population. -
Alternative names
- Cell Cytotoxicity
- cell tracking
Datasheets and documents
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Datasheet download
References (1)
ab176737 has been referenced in 1 publication.
- Jazayeri SD et al. HPV16-E7-Specific Activated CD8 T Cells in E7 Transgenic Skin and Skin Grafts. Front Immunol 8:524 (2017). PubMed: 28523003
