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Cell Tracking Dye Kit - Blue - Cytopainter (ab138890)

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  • SDS
  • Protocol Booklet
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    Overview

    • Product name

      Cell Tracking Dye Kit - Blue - Cytopainter
      See all Cell tracking kits
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Cell-based
    • Product overview

      Cell Tracking Dye Kit - Blue | Cytopainter (ab138890) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. Dye can be visualized with a blue filter (Ex/Em = 360/445 nm).


      The labelling process is robust, requiring minimal hands-on time. This product can be used in different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.The kit provides all the essential components with an optimized cell-labelling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

    • Notes

      Uniform labeling of live cells with a hydrophobic dye whose fluorescence is strongly enhanced upon entering the cell. Cellular esterases convert the substrate into a strong fluorescent photostable hydrophilic dye that is well retained in the cytoplasm. Cells need to be viewed in less than 24 - 48 hours as dye will not be passed to daughter cells.

      If you would like to track cells during several generations, we recommend CytoPainter Cell Proliferation Staining Reagent - Blue Fluorescence (ab176726).

    • Platform

      Microplate reader, Fluor. microscope, Flow cyt.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 200 tests
      Assay Buffer 1 x 20ml
      DMSO 1 x 0.2ml
      Tracking dye Blue 2 vials
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Staining Kits
      • Cell Tracking kits

    Associated products

      Images

      • Fluorescence Microscopy
        Fluorescence Microscopy
        Image of U2OS cells stained with 2 µM CytoPainter Cell Tracking Staining Kit - Blue Fluorescence in a black wall/clear bottom 96-well plate.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab138890? Please let us know so that we can cite the reference in this datasheet.

      ab138890 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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