Overview

  • Product name

    Cell Tracking Dye Kit - Deep Red - Cytopainter
    See all Cell tracking kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Product overview

    Cell Tracking Dye Kit - Deep Red | Cytopainter (ab138894) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. Dye can be visualized with a blue filter (Ex/Em = 630/650 nm).


    The labelling process is robust, requiring minimal hands-on time. This product can be used in different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.The kit provides all the essential components with an optimized cell-labelling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

  • Notes

    Uniform labeling of live cells with a hydrophobic dye whose fluorescence is strongly enhanced upon entering the cell. Cellular esterases convert the substrate into a strong fluorescent photostable hydrophilic dye that is well retained in the cytoplasm. Cells need to be viewed in less than 24 - 48 hours as dye will not be passed to daughter cells.

    If you would like to track cells during several generations, we recommend CytoPainter Cell Proliferation Staining Reagent - Deep Red Fluorescence (ab176736).

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

Images

  • Hela cells stained with CytoPainter Cell Tracking Staining Kit - Deep Red Fluorescence in a Costar black wall/clear bottom 96-well plate.

Protocols

References

ab138894 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Yes, it can be fixed. But the signal intensity might decrease after overnight fixation.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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