Overview

  • Product name

    Cell Tracking Dye Kit - Green - Cytopainter
    See all Cell tracking kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    Cell Tracking Dye Kit - Green | Cytopainter (ab138891) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. Dye can be visualized with a green filter (Ex/Em = 490/520 nm).


    The labelling process is robust, requiring minimal hands-on time. This product can be used in different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.The kit provides all the essential components with an optimized cell-labelling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

  • Notes

    Uniform labeling of live cells with a hydrophobic dye whose fluorescence is strongly enhanced upon entering the cell. Cellular esterases convert the substrate into a strong fluorescent photostable hydrophilic dye that is well retained in the cytoplasm. Cells need to be viewed in less than 24 - 48 hours as dye will not be passed to daughter cells.

    If you would like to track cells during several generations, we recommend CytoPainter Cell Proliferation Staining Reagent - Green Fluorescence (ab176735).

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

Images

  • Image of U2OS cells stained with CytoPainter Cell Tracking Staining Kit - Green Fluorescence in a black 96-well plate.

Protocols

References

This product has been referenced in:

  • Wierzbicki M  et al. Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion. Int J Nanomedicine 12:7241-7254 (2017). Read more (PubMed: 29042773) »
  • Hassanein W  et al. Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold. Organogenesis 13:16-27 (2017). Read more (PubMed: 28029279) »
See all 4 Publications for this product

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