Cell Tracking Dye Kit - Red - Cytopainter (ab138893)

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Overview

  • Product name

    Cell Tracking Dye Kit - Red - Cytopainter
    See all Cell tracking kits

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Cell-based

  • Product overview

    Cell Tracking Dye Kit - Red | Cytopainter (ab138893) is designed to uniformly label live cells for studies where multicolor analysis might be required, such as cell drug resistance, cell adhesion or chemotaxis. Dye can be visualized with a blue filter (Ex/Em = 575/600 nm).


    The labelling process is robust, requiring minimal hands-on time. This product can be used in different types of fluorescence platforms such as microplate assays, flow cytometry and fluorescence microscope.The kit provides all the essential components with an optimized cell-labelling protocol, and can be used for both proliferating and non-proliferating cells (either suspension or adherent cells).

  • Notes

    Uniform labeling of live cells with a hydrophobic dye whose fluorescence is strongly enhanced upon entering the cell. Cellular esterases convert the substrate into a strong fluorescent photostable hydrophilic dye that is well retained in the cytoplasm. Cells need to be viewed in less than 24 - 48 hours as dye will not be passed to daughter cells.

     

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

Images

  • Hela cells stained with CytoPainter Cell Tracking Staining Kit - Red Fluorescence
    Hela cells stained with CytoPainter Cell Tracking Staining Kit - Red Fluorescence
    Hela cells stained with CytoPainter Cell Tracking Staining Kit - Red Fluorescence in a Costar black wall/clear bottom 96-well plate

References

Publishing research using ab138893? Please let us know so that we can cite the reference in this datasheet.

ab138893 has been referenced in 1 publication.

  • Hassanein W  et al. Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold. Organogenesis 13:16-27 (2017). PubMed: 28029279

Customer reviews and Q&As

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Question

I have a problem with it because I observed a lot of red discrete points and not a diffuse staining of the cytoplasm. I think the staining is blocked in endosomes.
I would like to know if you already had feedback with this problem on primary cells? What can be the explanation?

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Answer


I have contacted the lab and they have confirmed that this staining pattern observed is to be expected because the dyes used in cell tracking kits can accumulate in any cell organelles such as cytoplasm (preferably), mitochondria, and lysosome etc. In this particular case, the dye has mostly accumulated in the lysosome. Other kits from other suppliers will differ in that the dye will accumulate in different organelles.

We are currently trying to develop a kit which uses a dye which accumulates predominantly in the cytosol which might be more useful to you. Alternatively, to see a more diffuse staining all over the cells as seen on our datasheets, a high concentration of the dye will need to be used.

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