For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
I have a problem with it because I observed a lot of red discrete points and not a diffuse staining of the cytoplasm. I think the staining is blocked in endosomes.
I would like to know if you already had feedback with this problem on primary cells? What can be the explanation?
Asked on May 09 2014
I have contacted the lab and they have confirmed that this staining pattern observed is to be expected because the dyes used in cell tracking kits can accumulate in any cell organelles such as cytoplasm (preferably), mitochondria, and lysosome etc. In this particular case, the dye has mostly accumulated in the lysosome. Other kits from other suppliers will differ in that the dye will accumulate in different organelles.
We are currently trying to develop a kit which uses a dye which accumulates predominantly in the cytosol which might be more useful to you. Alternatively, to see a more diffuse staining all over the cells as seen on our datasheets, a high concentration of the dye will need to be used.
Elisa ThomasAbcam Scientific Support
Answered on May 09 2014