Cell Transformation Assay Kit (Fluorometric) (ab235699)

Overview

  • Product name

    Cell Transformation Assay Kit (Fluorometric)
    See all Cell Transformation kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based (quantitative)
  • Species reactivity


    Predicted to work with: Mammals
  • Product overview

    Transformed cells can proliferate without attaching to a surface. Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). The Cell Transformation Assay Kit (Fluorimetric) (ab235699) is faster, stable and more sensitive than the traditional Soft-Agar Assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Agarose Powder 1 x 240mg
    Agarose Solubilization Solution 1 x 5ml
    DMEM Solution (10X) 2 x 1.5ml
    Quantitative Dye (200X) 1 x 0.1ml
    Staining Solution 1 x 1ml

Images

  • (a) HeLa (Human epithelial cell line from cervix adenocarcinoma) cell-dose curve; (b) Equal numbers of NIH/3T3 (Mouse embryo fibroblast cell line) and HeLa cells were serially diluted and seeded in agarose gel. Cells were solubilized and detected with Quantitative Dye; (c) Image of HeLa cell colonies. HeLa cells were cultured for 7 days according to the kit protocol.

Protocols

References

ab235699 has not yet been referenced specifically in any publications.

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