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I want to test for viability in cell culture supernatants. We are using RPMI + 10% FBS in our assay. Will this have an effect in the readout?
Asked on Feb 26 2013
You can use a control media with the same amount of RPMI and FBS to account for any signals generated due to these 2 in your sample readings. Just subtract the readings you get in the controls from the standards.
Please let me know if you have any further questions.
Answered on Feb 26 2013