Question (72419) | Cell Viability Assay Kit (Bioluminescent) (ab65314)

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Question

I want to test for viability in cell culture supernatants. We are using RPMI + 10% FBS in our assay. Will this have an effect in the readout?

Answer



I've confirmed with the lab, and you can use this kind of media but be sure to use a control media with the same amount of RPMI and FBS to account for any signals generated due to these 2 in your sample readings. Just subtract the control readings from the other readings.

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