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    cell-viability-assay-kit-fluorometric-dual-greenred-ab112121.pdf

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Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)

  • Datasheet
  • SDS
  • Protocol Booklet
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Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader, Fluor. microscope, Flow cyt.
    • Sample type: Adherent cells, Suspension cells

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    Overview

    • Product name

      Cell Viability Assay Kit (Fluorometric - Dual Green/Red)
      See all Cell Viability kits
    • Detection method

      Fluorescent
    • Sample type

      Adherent cells, Suspension cells
    • Assay type

      Quantitative
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. ab112121 uses two non-fluorescent indicators: The CellGreen fluorescent dye for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes. The non-fluorescent Green Indicator Dye can easily permeate intact live cells and is hydrolyzed by intracellular esterase to generate the strongly fluorescent hydrophilic CellGreen fluorescent dye which is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to the DNA of dead cells. Cells grown in black-wall plates can be stained and quantified in less than two hours.


      ab112121 is more robust and accurate than the other viability assays. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, fluorescence microscope, and flow cytometry. The kit provides all the essential components with an optimized assay protocol. It is suitable for both proliferating and non-proliferating cells (either suspension or adherent cells). ab112121 comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).

    • Notes

      ab112121 should be stored desiccated.Assay kit comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).

      Related assays

      Review the cell health assay guide to learn about kits to perform a cell viability assay, cytotoxicity assay and cell proliferation assay. 

    • Platform

      Microplate reader, Fluor. microscope, Flow cyt.

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components 2 x 96 tests
      Assay Buffer 1 x 20ml
      CellGreen fluorescent dye 2 vials
      DMSO 1 x 100µl
      Propidium Iodide 1 x 40µl
    • Research areas

      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Cell Viability and Senescence Kits
      • Kits/ Lysates/ Other
      • Kits
      • Apoptosis Kits
      • Apoptosis Assay Kits
      • Cell viability
      • Kits/ Lysates/ Other
      • Kits
      • Cell Damage Kits
      • Cell viability, plasma membrane damage
    • Relevance

      Cell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
    • Alternative names

      • cell tracking

    Images

    • Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)
      Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)
      CHO-K1 cell number response was measured with ab112121. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab112121? Please let us know so that we can cite the reference in this datasheet.

    ab112121 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Question

    We received a request of one of our customers. He likes a kit for bacteria viability, I found invitrogen L7007/L7012, but, in abcam there is ab112121 that is similar (I think).    I would like to know if you could send us a protocol for use in bacteria of ab112121 or could I suggest same protocol that Invitrogen?  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 22 2012

    Answer

    Thank you for contacting us.

    Yes, it should work theoretically. For dead cells, the dye is same in ab112121 and Invitrogen L-7007. For live cells, the working principle is different. Ab112121 is for labeling only live cells, Invitrogen's for both live and dead. You can use the same protocol as mammalian cells. see the link below.

    https://www.abcam.com/ps/products/112/ab112121/documents/ab112121%20Cell%20Viability%20Assay%20Kit%20(Fluorometric%20-%20Dual%20Green%20Red)%20v2%20(website).pdf

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Oct 22 2012

    Question

    I wonder if you have assays there proliferation and viability could be detected independently in the same well. (eg. two wave lengths one for proliferation and another for survival).

    Read More

    Abcam community

    Verified customer

    Asked on Aug 20 2012

    Answer

    Thank you for contacting us.

    Unfortunately, we do not have a kit which combines these two factors in one, or more precisely: we haven't tested them in combination. However, we do have an abundance of kits which can measure this two factors separately:

    https://www.abcam.com/Cell-Proliferation-Assay-Kit-Fluorometric-Blue-ab102501.html (or use the following: https://www.abcam.com/Cell-Proliferation-Assay-Kit-Fluorometric-Blue-ab102501.html).

    https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Dual-GreenRed-ab112121.html (or use the following: https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Dual-GreenRed-ab112121.html).



    I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 20 2012

    Question

    What are the names of the fluorophores in the kits ab112121 and ab112122 ?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 22 2012

    Answer



    Les filtres à utiliser sont FITC (vert)et Cy3ou Texas red (rouge).

    Read More

    Abcam Scientific Support

    Answered on Jun 22 2012

    Question

    I´m interested in your Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121) . You mentioned in your assay that esterase activity is propotrtional to the viability of the cells. Could you please provide a reference for me regarding this sentence?

    Read More

    Abcam community

    Verified customer

    Asked on May 31 2012

    Answer

    Thank you for contacting us.

    To our knowledge, this kit has not yet been cited in any publications. While the specifics of the dyes used in this kit are considered proprietary, the product overview provides a general description about how the assay works:

    "The non-fluorescent Green Indicator Dye can easily permeate intact live cells and is hydrolyzed by intracellular esterase to generate the strongly fluorescent hydrophilic Green Indicator Dye which is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to the DNA of dead cells."

    The graph on our datasheet also shows an experiment where the fluorescent signal was shown to be linearly proportional to the number of live cells in the well. If you need any more specific information, please let me know and I will be happy to help you further.

    Read More

    Abcam Scientific Support

    Answered on May 31 2012

    Question

    Hi,

    I am using Greiner Thincert Culture Inserts for 6-well plates and A549 cells in my exposure experiments. The goal is to expose the cells at an air/liquid interphase for a certain period and to measure cell viability, oxidative stress markers and cell cycle stage after the exposure.

    Until now I have tried to detach the cells from the inserts and measure the MTT assay and do flow cytometry analysis. However I have been having problems detaching the cells from the inserts, trypsin/EDTA is ineffective and scraping destroys the cells, so I have not been able to get sensible data from our exposure experiments.

    Can you recommend any kits/procedures which could be applied directly in the inserts without prior detachments of the cells?

    The minimum parameters I would need to evaluate after the exposure are cell viability and apoptosis/cell cycle stage, but I would like to include a marker for oxidative stress (e.g. glutathione) as well. I should be able to measure all the mentioned parameters simultaneously in one insert without too much quenching and fluorescence interference/overlay.

    We have a Victor III microplate reader and an inverted fluorescence microscope for which we will acquire quantification software shortly.

    Thank you for your help.

    With best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Mar 06 2012

    Answer

    Thank you for your enquiry and interest in our products.

    MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the cells in the wells of the 96 well plates, then apply the MTT solution (20ul) to each well. During incubation (37C, 5% CO2) for 1-5 hours MTT will be metabolized. The formazan (MTT metabolic product) can then be dissolved in DMSO and the optical density can be read at 560 nm using a plate reader - without harvesting the cells. There may be other cell viability/cytotoxicyprotovols but this is the most widely used.

    Cell viability kits:

    We have different cell viability assay kits in our catalogue i.e. ab112120, ab112121, ab112122, ab112123.

    https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Blue-ab112120.html

    https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Dual-Green-Red-ab112121.html

    https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Green-ab112122.html

    https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Near-InfraRed-ab112123.html

    GSH kits:

    GSH can be detected/quantified either by fluorimetric or chromogenic (DTNB - Ellman's reagent) assays and currently we have also some assay kits for detecting this tripeptide, you may wish to take a look at the datasheets for further information.

    - ab65322: Glutathione Fluorometric Detection Kit

    https://www.abcam.com/Glutathione-Fluorometric-Detection-Kit-ab65322.html

    - ab112132:Intracellular GSH Assay Kit

    https://www.abcam.com/Intracellular-GSH-Assay-Kit-ab112132.html

    Normally, intracellular GSH levels can be expressed per cell numbers or per protein concentration.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Mar 06 2012

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