Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameCell Viability Assay Kit (Fluorometric - Dual Green/Red)
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Sample typeAdherent cells, Suspension cells
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Assay typeQuantitative
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Species reactivityReacts with: Other species, Mammals
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Product overview
Abcam's Cell Viability assay kits are a set of tools for monitoring cell viability and cellular functions. ab112121 uses two non-fluorescent indicators: The CellGreen fluorescent dye for viable cells and a cell-impermeable DNA-binding dye for the cells with compromised membranes. The non-fluorescent Green Indicator Dye can easily permeate intact live cells and is hydrolyzed by intracellular esterase to generate the strongly fluorescent hydrophilic CellGreen fluorescent dye which is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to the DNA of dead cells. Cells grown in black-wall plates can be stained and quantified in less than two hours.
ab112121 is more robust and accurate than the other viability assays. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, fluorescence microscope, and flow cytometry. The kit provides all the essential components with an optimized assay protocol. It is suitable for both proliferating and non-proliferating cells (either suspension or adherent cells). ab112121 comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).
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Notes
ab112121 should be stored desiccated.Assay kit comes with sufficient reagents to perform either 200 assays (96-well format) or 800 assays (a 384-well format).
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Tested applicationsSuitable for: Flow Cytmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 2 x 96 tests Assay Buffer 1 x 20ml CellGreen fluorescent dye 2 vials DMSO 1 x 100µl Propidium Iodide 1 x 40µl -
Research areas
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RelevanceCell viability is a determination of living or dead cells, based on a total cell population. Cell viability assess healthy cells in a sample, with no distinction between dividing or quiescent cells. An increase in cell viability indicates cell growth, while a decrease in viability can generally be interpreted as the result of either toxic effects of compounds/agents or suboptimal culture conditions.
Applications
Our Abpromise guarantee covers the use of ab112121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
Images
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Functional Studies - Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121)CHO-K1 cell number response was measured with ab112121. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).
Protocols
References
ab112121 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"