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I´m interested in your Cell Viability Assay Kit (Fluorometric - Dual Green/Red) (ab112121) . You mentioned in your assay that esterase activity is propotrtional to the viability of the cells. Could you please provide a reference for me regarding this sentence?
Asked on May 31 2012
Thank you for contacting us.
To our knowledge, this kit has not yet been cited in any publications. While the specifics of the dyes used in this kit are considered proprietary, the product overview provides a general description about how the assay works:
"The non-fluorescent Green Indicator Dye can easily permeate intact live cells and is hydrolyzed by intracellular esterase to generate the strongly fluorescent hydrophilic Green Indicator Dye which is well-retained in the cell cytoplasm. The esterase activity is proportional to the number of viable cells. The DNA-binding dye is quite polar and impermeable for viable cells that have intact membranes. It becomes fluorescent only upon binding to the DNA of dead cells."
The graph on our datasheet also shows an experiment where the fluorescent signal was shown to be linearly proportional to the number of live cells in the well. If you need any more specific information, please let me know and I will be happy to help you further.
Answered on May 31 2012