Overview

  • Product name

    Cell Viability Assay Kit (Fluorometric - Green)
    See all Cell Viability kits
  • Detection method

    Fluorescent
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Quantitative
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    There are a variety of parameters that can be used to monitor cell viability. The green fluorescent dye used in the kit is a hydrophobic compound. It easily permeates intact live cells and gets enhanced fluorescence upon entering into live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strongly green fluorescent hydrophilic product that is well-retained in the cell cytoplasm. The green fluorophore generated by the non-fluorescent substrate used in the kit has the spectral properties of fluorescein at Ex/Em = ~490 nm/520 nm. When well excited with the Argon Laser at 488 nm, the fluorophore emits intense green fluorescence at ~520 nm.


    ab112122 provides all the essential components with an optimized cell-labeling protocol for fluorescence microplate assays. It can also be used with a fluorescence microscope equipped with a FITC filter set.


    ab112122 provides an effective tool of labeling cells for fluorescence microplate and microscopic investigations of cellular functions. It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. ab112122 is suitable for proliferating and non-proliferating cells.

    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Review the cell health assay guide to learn about more kits to perform a cell viability assaycytotoxicity assay and cell proliferation assay

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

Images

  • CHO-K1 cell number response was measured with ab112122. CHO-K1 cells at 0 to 5,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 100 µL/well of Green dye-loading solution for 1 hour at 37 °C. The fluorescence intensity was measured at Ex/Em = 490/ 525 nm. The fluorescence intensity was linear (R2 = 1) to the cell number as indicated. The detection limit was 30 cells/well (n=6).

Protocols

References

ab112122 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer



Les filtres à utiliser sont FITC (vert)et Cy3ou Texas red (rouge).

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Answer

Thank you for your enquiry and interest in our products.

MTT assay can be carried out on 96 well plates using either adherent or floating cells. You do not necessary need to remove (harvest) the cells by trypsinization. You can culture the cells in the wells of the 96 well plates, then apply the MTT solution (20ul) to each well. During incubation (37C, 5% CO2) for 1-5 hours MTT will be metabolized. The formazan (MTT metabolic product) can then be dissolved in DMSO and the optical density can be read at 560 nm using a plate reader - without harvesting the cells. There may be other cell viability/cytotoxicyprotovols but this is the most widely used.

Cell viability kits:

We have different cell viability assay kits in our catalogue i.e. ab112120, ab112121, ab112122, ab112123.

https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Blue-ab112120.html

https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Dual-Green-Red-ab112121.html

https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Green-ab112122.html

https://www.abcam.com/Cell-Viability-Assay-Kit-Fluorometric-Near-InfraRed-ab112123.html

GSH kits:

GSH can be detected/quantified either by fluorimetric or chromogenic (DTNB - Ellman's reagent) assays and currently we have also some assay kits for detecting this tripeptide, you may wish to take a look at the datasheets for further information.

- ab65322: Glutathione Fluorometric Detection Kit

https://www.abcam.com/Glutathione-Fluorometric-Detection-Kit-ab65322.html

- ab112132:Intracellular GSH Assay Kit

https://www.abcam.com/Intracellular-GSH-Assay-Kit-ab112132.html

Normally, intracellular GSH levels can be expressed per cell numbers or per protein concentration.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for contacting us regarding this issue. I have spoken with the lab about you results using ab112122. It seems from your results that most likely you have used the top read mode. For this assay, it must use bottom read mode for measuring, and also use cutoff filter at 515nm if using Flexstation from Molecular Devices. We hope this helps you. Please do not hesitate to contact us if you have other questions or if there are more ways that we may help you reach your research goals.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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