Overview

  • Product name
  • Description
    Rabbit polyclonal to Cellubrevin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Cellubrevin aa 1-18.
    Sequence:

    MSTGVPSGSSAATGSNRR


    (Peptide available as ab5861)

  • Positive control
    • In Western Blot HeLa cell extract (ab7898) can be used as a positive control.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer’s disease, all VAMP isoform levels appear to be significantly reduced.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).

Target

Images

  • Western blot analysis of recombinant rat VAMP-3 using ab5789.

  • Western blot analysis of VAMP-3 in HeLa cell extract using ab5789.

References

This product has been referenced in:
  • Verboogen DRJ  et al. Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules. FEBS Lett 592:1535-1544 (2018). Read more (PubMed: 29570778) »
  • Verboogen DRJ  et al. Fluorescence Lifetime Imaging Microscopy reveals rerouting of SNARE trafficking driving dendritic cell activation. Elife 6:N/A (2017). WB, ICC/IF ; Human . Read more (PubMed: 28524818) »
See all 3 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (4-20 % gradient gel)
Sample
Human Cell lysate - whole cell (HeLa, U266)
Specification
HeLa, U266
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 13 2013

Answer

Thank you for your note. I am sorry to hear that even the replacement vial failed to work. This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab68833. For your information, the internal reference note for this credit is CN19268. You can use this credit in the near future for any of the products which are in the catalogue. I hope this helps and if I can assist further, please do not hesitate to contact me.

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Answer

Thank you for your response and for reattaching the image. I can arrange the credit note for the cost of ab68833, our Abpromise applies for this complaint. Initially, the problem was reported only with ab68833 and looking at our system there is no record for a complaint for the other antibody ab81610. Every single complaint needs to be reported and investigated independently. As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased. Regarding ab81610: - Could you please confirm the batch and order number, - Please specify the secondary antibody used for detection I look forward to hearing from you soon.

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Answer

Thank you for your new contact and updates. Unfortunately, one the files you have kindly sent to us can’t be opened so I am afraid I am unable to take a look at it. Could you resend it to me? According to our last correspondence (16th December 2011), you did not receive the replacement vial of this antibody which was sent to your Company on 1st December. - When you received the replacement vial finally? - How was the antibody stored? I understand that you are satisfied with another Abcam product (ab5789) which worked as it expected. I could arrange a credit note for you for the cost of the original ab68833 which you can use in future purchase. Let me know how you wish to proceed. I look forward to hearing from you soon.

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Answer

Thank you for your email. Ab5789 was characterized for use in Western blotting and was not tested in other applications. By Western blot, the antibody detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa) representing recombinant rat VAMP-3. The immunogen used to generate this antibody was a synthetic peptide: STGVPSGSSAATGSNRRC, corresponding to amino acids 1-18 of Mouse VAMP3. Ab5789 has not been tested for cross-reactivity with the other VAMP isoforms. However, if you BLAST the peptide sequence used to generate this antibody (the ExPASy and NCBI links are on the online datasheet) there is no identity with other VAMPs. We do have a review from a customer who used this antibody in WB and IF (you can read her comments by clicking on the Abreviews tab located on the online product datasheet). She states that the antibody worked well for WB, but states "I expected the VAMP3 AB to have a more localized perinuclear staining, but the staining of this AB is homogeneous throughout the entire cell." It does seem that this antibody may not be suitable for IF. I do suggest that you submit an Abreview via the product datasheet. We always encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. I hope this helps. Please contact us again if you have any additional questions.

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Question
Answer

Thank you for your enquiry. Ab5789 has not been tested for cross-reactivity with the other VAMP isoforms. However, if you BLAST the peptide sequence used to generate this antibody (the ExPASy and NCBI links are on the online datasheet) there is no identity with other VAMPs. I hope this helps, if you have any additional questions please contact us again.

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Answer

Regarding ab2102 and ab2103, unfortunately the peptide sequences are not available. If you have any additional questions, please let us know.

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Answer

Thank you for your enquiry. Cellubrevin is an alternative name for VAMP3, and so these 3 antibodies target the same protein. Ab2103 has been tested for cross-reactivity with Human and Mouse, and does not cross-react with Rat or Dog. Ab2102 cross-reacts with Rat and does not cross-react with Human or Dog. Ab5789 cross-reacts with Dog, Human, Mouse and Rat. So none have been tested yet for cross-reactivity with guinea pig, to our knowledge. I will try to obtain the peptide sequences for ab2102 and ab2103 and will let you know.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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