Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (4-20 % gradient gel)
Sample
Human Cell lysate - whole cell (HeLa, U266)
Specification
HeLa, U266
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Sigma A0545 anti-IgG Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
WesternBright SIRIUS Substrate Kit
Bands
Specific: around 15 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
HeLa cells
Abcam user community
Verified customer
Submitted Dec 13 2013