Overview

  • Product name

    Anti-Cellubrevin antibody [EPR16866]
    See all Cellubrevin primary antibodies
  • Description

    Rabbit monoclonal [EPR16866] to Cellubrevin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein within Human Cellubrevin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: Q15836

  • Positive control

    • WB: Human fetal lung and placenta lysates; A-375 whole cell lysate. ICC/IF: A-375 and A549 cells. Flow Cyt: A-375 cells. IP: A-375 whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
ICC/IF 1/500.
Flow Cyt 1/60.
IP 1/30.

Target

  • Function

    SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • CEB antibody
    • Cellubrevin antibody
    • Synaptobrevin 3 antibody
    • Synaptobrevin-3 antibody
    • VAMP 3 antibody
    • VAMP-3 antibody
    • VAMP3 antibody
    • VAMP3_HUMAN antibody
    • Vesicle associated membrane protein 3 antibody
    • Vesicle-associated membrane protein 3 antibody
    see all

Images

  • Anti-Cellubrevin antibody [EPR16866] (ab200657) at 1/2000 dilution + Human fetal lung lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Cellubrevin antibody [EPR16866] (ab200657) at 1/10000 dilution + A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Cellubrevin antibody [EPR16866] (ab200657) at 1/10000 dilution + Human placenta lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A-375 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling Cellubrevin with ab200657 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasm staining on A549 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab200657 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling Cellubrevin with ab200657 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Cellubrevin was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab200657 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200657 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
    Lane 1: A-375 whole cell lysate 10µg (Input).
    Lane 2: ab200657 IP in A-375 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200657 in A-375 whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

     

References

ab200657 has not yet been referenced specifically in any publications.

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