Key features and details
- Detection method: Fluorescent
- Sample type: Adherent cells
Product nameCellular Antioxidant Assay Kit
See all Oxidative Stress kits
Sample typeAdherent cells
Cellular Antioxidant Assay Kit (ab242300) is a cell-based assay for measuring antioxidant activity within adherent cells.
Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
Fluorescence is measured over time in a standard microplate fluorometer. This fluorescence correlates to the Quercetin’s ability to quench free radicals. Test antioxidant values can be compared to Quercetin to determine antioxidant activity within the cell.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 192 tests 96-Well Cell Culture Microtiter Plate 2 units DCFH-DA Probe (1000X) 1 x 100µl Free Radical Initiator 1 x 200mg Quercetin 1 x 250µl
ab242300 has not yet been referenced specifically in any publications.