Key features and details
- Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cellular Apoptosis Susceptibility/CSE1L antibody
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies
DescriptionRabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
Synthetic peptide corresponding to Human Cellular Apoptosis Susceptibility/CSE1L (N terminal).
Database link: NP_001307.2
- WB: HeLa and Ramos whole cell lysate. IP: HEK-293T whole cell lysate. IHC-P: Human testicular seminoma; mouse plasmacytoma.
This product was previously labelled as Cellular Apoptosis Susceptibility
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionExport receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Tissue specificityHighly expressed in proliferating cells.
Sequence similaritiesBelongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab70547) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from Ramos cells at 50 µg
Developed using the ECL technique.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes
Paraffin embedded mouse plasmacytoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.
Paraffin embedded human testicular seminoma tissue stained for CSE1L using ab70547 at 0.2 µg/ml in immunohistochemical analysis.
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK-293T cells prepared using NETN lysis buffer.
Affinity purified rabbit anti-CSE1 antibody used for IP at 6 µg per reaction. CSE1 was also immunoprecipitated by a previous lot of this antibody and rabbit anti-CSE1 antibody BL1977.
For blotting immunoprecipitated CSE1, ab70547 was used at 1 µg/ml. Chemiluminescence had an exposure time of 10 seconds.
ab70547 has not yet been referenced specifically in any publications.