Overview

  • Product name

    Anti-Cellular Apoptosis Susceptibility/CSE1L antibody
    See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies
  • Description

    Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human Cellular Apoptosis Susceptibility/CSE1L (N terminal).
    Database link: NP_001307.2

  • Positive control

    • Whole cell lysate from HeLa and Ramos cells
  • General notes

     This product was previously labelled as Cellular Apoptosis Susceptibility

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/25000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
  • Tissue specificity

    Highly expressed in proliferating cells.
  • Sequence similarities

    Belongs to the XPO2/CSE1 family.
    Contains 1 importin N-terminal domain.
  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAS antibody
    • Cellular apoptosis susceptibility protein antibody
    • Chromosome segregation 1 (yeast homolog) like antibody
    • Chromosome segregation 1 Like antibody
    • Chromosome segregation 1 like protein antibody
    • Chromosome segregation 1-like protein antibody
    • Chromosome segregation gene CSE1 antibody
    • CSE 1 antibody
    • CSE 1 chromosome segregation 1 like antibody
    • CSE 1 chromosome segregation 1 like protein antibody
    • CSE 1L antibody
    • CSE1 antibody
    • CSE1 chromosome segregation 1 like (yeast) antibody
    • CSE1 chromosome segregation 1 like antibody
    • CSE1 chromosome segregation 1 like protein antibody
    • CSE1L antibody
    • Exp 2 antibody
    • Exp2 antibody
    • Exportin 2 antibody
    • Exportin-2 antibody
    • Exportin2 antibody
    • Importin alpha re exporter antibody
    • Importin-alpha re-exporter antibody
    • MGC117283 antibody
    • MGC130036 antibody
    • MGC130037 antibody
    • XPO 2 antibody
    • XPO2 antibody
    • XPO2_HUMAN antibody
    see all

Images

  • All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab70547) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from Ramos cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 110 kDa
    Observed band size: 110 kDa


    Exposure time: 3 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) labelling Cellular Apoptosis Susceptibility/CSE1L with ab70547 at 1/5000 (0.2µg/ml) and 1/1000 (1µg/ml). Detection: DAB.

  • 1mg whole cell lysate was immunoprecipitated using ab70547 at 3ug/ml (lane 1) or a control IgG (lane 2). For the subsequent blot 25% of the immunoprecipitate was loaded per lane and probed with ab70547 at 1ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.

References

ab70547 has not yet been referenced specifically in any publications.

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