Product nameAnti-Cellular Apoptosis Susceptibility/CSE1L antibody
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies
DescriptionRabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
Recombinant fragment corresponding to Human Cellular Apoptosis Susceptibility/CSE1L aa 222-439.
Database link: NP_001307)
- 293T, A431, H1299, HeLa whole cell lysate (ab150035). HepG2, Molt-4 and Raji Lysates. Cal27 Xenograft tissue.
This product was previously labelled as Cellular Apoptosis Susceptibility
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 110 kDa.|
|IHC-P||1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH 8.0)|
|ICC/IF||1/100 - 1/200.|
FunctionExport receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Tissue specificityHighly expressed in proliferating cells.
Sequence similaritiesBelongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96755 (1) and merged with DNA probe (2) at 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using ab96755 at 1/500 dilution.
All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/2500 dilution
Lane 1 : Hela extracts 25 ug
Lane 2 : RPE-1 extracts 25 ug
Lane 3 : Hela extracts 50 ug
Lane 4 : RPE-1 extracts 50 ug
All lanes : Donkey LICOR anti-rabbit IRDye680 at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cross reactivity), 70 kDa (possible cross reactivity)
Exposure time: 3 minutes
This product has been referenced in:
- Ma S et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging (Albany NY) 10:2062-2078 (2018). Read more (PubMed: 30144787) »
- Gagné B et al. Importin ß1 targeting by hepatitis C virus NS3/4A protein restricts IRF3 and NF-?B signaling of IFNB1 antiviral response. Traffic 18:362-377 (2017). Read more (PubMed: 28295920) »