Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Cellular Apoptosis Susceptibility/CSE1L antibody
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies -
Description
Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Zebrafish
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Immunogen
Recombinant fragment corresponding to Human Cellular Apoptosis Susceptibility/CSE1L aa 222-439.
Database link: NP_001307) -
Positive control
- 293T, A431, H1299, HeLa whole cell lysate (ab150035). HepG2, Molt-4 and Raji Lysates. Cal27 Xenograft tissue.
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General notes
This product was previously labelled as Cellular Apoptosis Susceptibility
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab96755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 110 kDa. | |
| IHC-P | 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH 8.0) | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Tissue specificity
Highly expressed in proliferating cells. -
Sequence similarities
Belongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 518622 Cow
- Entrez Gene: 1434 Human
- Entrez Gene: 110750 Mouse
- Entrez Gene: 362273 Rat
- Entrez Gene: 30707 Zebrafish
- Omim: 601342 Human
- SwissProt: P55060 Human
- SwissProt: Q9ERK4 Mouse
see all -
Alternative names
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
see all
Images
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Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab96755 (1) and merged with DNA probe (2) at 1/200 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using ab96755 at 1/500 dilution. -
Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/2500 dilution
Lane 1 : Hela extracts 25 ug
Lane 2 : RPE-1 extracts 25 ug
Lane 3 : Hela extracts 50 ug
Lane 4 : RPE-1 extracts 50 ug
Secondary
All lanes : Donkey LICOR anti-rabbit IRDye680 at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible cross reactivity), 70 kDa (possible cross reactivity)
Exposure time: 3 minutes
Protocols
References
This product has been referenced in:
- Ma S et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging (Albany NY) 10:2062-2078 (2018). Read more (PubMed: 30144787) »
- Gagné B et al. Importin ß1 targeting by hepatitis C virus NS3/4A protein restricts IRF3 and NF-?B signaling of IFNB1 antiviral response. Traffic 18:362-377 (2017). Read more (PubMed: 28295920) »
