Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)
Key features and details
- Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-Cellular Apoptosis Susceptibility/CSE1L antibody
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies -
Description
Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Zebrafish
-
Immunogen
Recombinant fragment corresponding to Human Cellular Apoptosis Susceptibility/CSE1L aa 222-439.
Database link: NP_001307 -
Positive control
- WB: HEK-293T, A431, H1299, HeLa whole cell lysate. PC-12 and Rat2 whole cell lysate. NIH/3T3, JC, BCL-1 and C2C12 whole cell lysate. IHC-P: Cal27 Xenograft. ICC/IF: HeLa cells.
-
General notes
This product was previously labelled as Cellular Apoptosis Susceptibility
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab96755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/20000. Predicted molecular weight: 110 kDa. | |
| IHC-P | 1/100 - 1/2000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH 8.0) |
|
| ICC/IF | 1/100 - 1/1000. |
Target
-
Function
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Tissue specificity
Highly expressed in proliferating cells. -
Sequence similarities
Belongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 518622 Cow
- Entrez Gene: 1434 Human
- Entrez Gene: 110750 Mouse
- Entrez Gene: 362273 Rat
- Entrez Gene: 30707 Zebrafish
- Omim: 601342 Human
- SwissProt: P55060 Human
- SwissProt: Q9ERK4 Mouse
see all -
Alternative names
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)
HeLa cells stained for CSE1L (green) using ab96755 at 1/2000 dilution in ICC/IF.
-
Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/10000 dilution
Lane 1 : PC-12 whole cell lysate
Lane 2 : Rat2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa7.5% SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96755 at 1/500 dilution.
-
Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa -
Western blot - Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755)All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody (ab96755) at 1/10000 dilution
Lane 1 : NIH/3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa7.5% SDS-PAGE
Protocols
References (5)
ab96755 has been referenced in 5 publications.
- Hu X et al. LncRNA Oprm1 overexpression attenuates myocardial ischemia/reperfusion injury by increasing endogenous hydrogen sulfide via Oprm1/miR-30b-5p/CSE axis. Life Sci 254:117699 (2020). PubMed: 32437793
- Ma S et al. LncRNA BANCR promotes tumorigenesis and enhances adriamycin resistance in colorectal cancer. Aging (Albany NY) 10:2062-2078 (2018). PubMed: 30144787
- Tian C et al. Multiplexed Thiol Reactivity Profiling for Target Discovery of Electrophilic Natural Products. Cell Chem Biol 24:1416-1427.e5 (2017). PubMed: 28988947
- Gagné B et al. Importin ß1 targeting by hepatitis C virus NS3/4A protein restricts IRF3 and NF-?B signaling of IFNB1 antiviral response. Traffic 18:362-377 (2017). PubMed: 28295920
- Sugiyama K et al. Calpain-Dependent Degradation of Nucleoporins Contributes to Motor Neuron Death in a Mouse Model of Chronic Excitotoxicity. J Neurosci 37:8830-8844 (2017). PubMed: 28821644
