Recombinant Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10327(B)] to Cellular Apoptosis Susceptibility/CSE1L
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)]
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies -
Description
Rabbit monoclonal [EPR10327(B)] to Cellular Apoptosis Susceptibility/CSE1L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Cellular Apoptosis Susceptibility/CSE1L aa 900 to the C-terminus. The exact sequence is proprietary.
Database link: P55060 -
Positive control
- SW480, MOLT4, HeLa, 293T, Ramos and MCF7 cell lysates; Human thyroid carcinoma tissue; SW480 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10327(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 110 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. -
Tissue specificity
Highly expressed in proliferating cells. -
Sequence similarities
Belongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1434 Human
- Entrez Gene: 110750 Mouse
- Omim: 601342 Human
- SwissProt: P55060 Human
- SwissProt: Q9ERK4 Mouse
- Unigene: 90073 Human
- Unigene: 22417 Mouse
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Alternative names
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
see all
Images
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution
Lane 1 : HeLa cell lysate at 20 µg
Lane 2 : HeLa Pulldown with ab70547 cell lysate at 10 µl
Lane 3 : HeLa Pulldown with ab172730 cell lysate at 10 µl
Performed under reducing conditions.
Predicted band size: 110 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] staining at 1/1000 dilution, shown in black. In Western blot, ab151546 was shown to bind specifically to Cellular Apoptosis Susceptibility/CSE1L. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 2 minutes exposure time. Secondary antibodies used were VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/10000 dilution.
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All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lane 5 : Ramos cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa -
Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of SW480 cells labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/250 dilution.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151546 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab151546 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151546 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151546 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151546 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151546 has been referenced in 2 publications.
- Gong Y et al. Age-Associated Proteomic Signatures and Potential Clinically Actionable Targets of Colorectal Cancer. Mol Cell Proteomics 20:100115 (2021). PubMed: 34129943
- Li Y et al. Elucidating the Host Interactome of EV-A71 2C Reveals Viral Dependency Factors. Front Microbiol 10:636 (2019). PubMed: 31001221