Recombinant
RabMAb

Recombinant Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546)

Overview

  • Product name

    Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)]
    See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies
  • Description

    Rabbit monoclonal [EPR10327(B)] to Cellular Apoptosis Susceptibility/CSE1L
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Cellular Apoptosis Susceptibility/CSE1L aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: P55060

  • Positive control

    • SW480, MOLT4, HeLa, 293T, Ramos and MCF7 cell lysates; Human thyroid carcinoma tissue; SW480 cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as Cellular Apoptosis Susceptibility

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
    • Tissue specificity

      Highly expressed in proliferating cells.
    • Sequence similarities

      Belongs to the XPO2/CSE1 family.
      Contains 1 importin N-terminal domain.
    • Cellular localization

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • CAS antibody
      • Cellular apoptosis susceptibility protein antibody
      • Chromosome segregation 1 (yeast homolog) like antibody
      • Chromosome segregation 1 Like antibody
      • Chromosome segregation 1 like protein antibody
      • Chromosome segregation 1-like protein antibody
      • Chromosome segregation gene CSE1 antibody
      • CSE 1 antibody
      • CSE 1 chromosome segregation 1 like antibody
      • CSE 1 chromosome segregation 1 like protein antibody
      • CSE 1L antibody
      • CSE1 antibody
      • CSE1 chromosome segregation 1 like (yeast) antibody
      • CSE1 chromosome segregation 1 like antibody
      • CSE1 chromosome segregation 1 like protein antibody
      • CSE1L antibody
      • Exp 2 antibody
      • Exp2 antibody
      • Exportin 2 antibody
      • Exportin-2 antibody
      • Exportin2 antibody
      • Importin alpha re exporter antibody
      • Importin-alpha re-exporter antibody
      • MGC117283 antibody
      • MGC130036 antibody
      • MGC130037 antibody
      • XPO 2 antibody
      • XPO2 antibody
      • XPO2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody [EPR10327(B)] (ab151546) at 1/1000 dilution

      Lane 1 : SW480 cell lysate
      Lane 2 : MOLT4 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : Ramos cell lysate
      Lane 6 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 110 kDa

    • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/50 dilution.

    • Immunofluorescent analysis of SW480 cells labeling Cellular Apoptosis Susceptibility/CSE1L with ab151546 at 1/250 dilution.

    • Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab151546 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Endometrial carcinoma tissue using ab151546 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151546 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab151546 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151546 showing +ve staining.

    References

    This product has been referenced in:

    See 1 Publication for this product

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