Key features and details
- Rabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L - N-terminal
- Suitable for: WB, IHC-P, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cellular Apoptosis Susceptibility/CSE1L antibody - N-terminal
See all Cellular Apoptosis Susceptibility/CSE1L primary antibodies
DescriptionRabbit polyclonal to Cellular Apoptosis Susceptibility/CSE1L - N-terminal
Tested applicationsSuitable for: WB, IHC-P, IP, ICC/IFmore details
Species reactivityReacts with: Human
- Human testis tissue; HeLa cells; 293 cell extracts.
This product was previously labelled as Cellular Apoptosis Susceptibility
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
PBS without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 110 kDa.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/100 - 1/500.|
FunctionExport receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Tissue specificityHighly expressed in proliferating cells.
Sequence similaritiesBelongs to the XPO2/CSE1 family.
Contains 1 importin N-terminal domain.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
- Information by UniProt
- CAS antibody
- Cellular apoptosis susceptibility protein antibody
- Chromosome segregation 1 (yeast homolog) like antibody
All lanes : Anti-Cellular Apoptosis Susceptibility/CSE1L antibody - N-terminal (ab189180) at 1/500 dilution
Lane 1 : 293 cell extract
Lane 2 : 293 cell extract with immunizing peptide
Predicted band size: 110 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling Cellular Apoptosis Susceptibility/CSE1L with ab189180 at 10 µg/ml.
Immunofluorescent analysis of HeLa cells labeling Exportin 2 with ab189180 at 1/100 dilution in the presence (right panel) or absence (left panel) of immunizing peptide.
ab189180 has not yet been referenced specifically in any publications.