For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    cellular-ros-assay-kit-orange-ab186028.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Kits/ Lysates/ Other Kits Cell Damage Kits Oxidative stress
Share by email

Cellular ROS Assay Kit (Orange) (ab186028)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question References (3)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Detection of ROS in Jurkat cells
  • Detection of ROS in Hela cells.
  • Hela cells stained with the Cellular Reactive Oxygen Species Detection Assay Kit (Orange Fluorescence) (ab186028)

Key features and details

  • Platform: Microplate reader, Fluor. microscope, Flow cyt.
  • Sample type: Adherent cells, Suspension cells

You may also be interested in

Dye
Product image
Lysosomal Staining Kit - Blue Fluorescence - Cytopainter (ab112135)
Assay
Product image
DCFDA / H2DCFDA - Cellular ROS Assay Kit (ab113851)
Biochemical
Product image
Dihydrorhodamine 123, Fluorescent probe for ROS (ab145260)

View more associated products

Overview

  • Product name

    Cellular ROS Assay Kit (Orange)
    See all Oxidative Stress kits
  • Sample type

    Adherent cells, Suspension cells
  • Product overview

    Cellular ROS Assay Kit (Orange) ab186028 uses an ROS sensor to quantify ROS in live cells. The orange dye used in the ROS assay protocol is cell-permeable and generates orange fluorescence when it reacts with ROS. The fluorescence signal of the dye can be measured by fluorescence microscopy, high-content imaging, microplate fluorometry, or flow cytometry.


    ab186028 provides a sensitive fluorometric, one-step assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format using either a fluorescence microplate reader at Ex/Em = 540/570 nm or a fluorescent microscope with TRITC filter.

  • Notes

    Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Orange Fluorescence).

    Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.

    Related products

    Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

    To measure reactive oxygen species within cells, we recommend DCFDA / H2DCFDA - Cellular ROS Assay Kit ab113851. Alternative ROS assays are available in orange (ab186028), red (ab186027), and deep red (ab186029).  ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates. 

    For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use ab139473; to assay superoxides use ab219943.

  • Platform

    Microplate reader, Fluor. microscope, Flow cyt.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 200 tests
    Assay Buffer 1 x 20ml
    DMSO 1 x 100µl
    ROS Orange Dye (Lyophilized) 1 vial
  • Research areas

    • Kits/ Lysates/ Other
    • Kits
    • Cell Damage Kits
    • Oxidative stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress
    • Kits/ Lysates/ Other
    • Kits
    • Cell Metabolism Kits
    • Oxidative Stress Assay Kits
    • Oxidative Stress

Images

  • Detection of ROS in Jurkat cells
    Detection of ROS in Jurkat cells

    Jurkat cells were treated without (Blue) or with 100µM TBHP (Red) for 30min at 37 °C, and then loaded with ROS orange in a 5% CO2, 37 °C incubator for 1 hour. The fluorescent intensities were measured with a flow cytometer using FL2 channel

  • Detection of ROS in Hela cells.
    Detection of ROS in Hela cells.

    Hela cells were seeded overnight at 15,000 cells/90 µl/well in a black wall/clear bottom 96-well plate. The cells were untreated (control) or treated with1 mM H2O2 or 100 µM TBHP for 30 minutes at 37 °C. The ROS Orange assay solution (100 µl/well) was added and incubated in a 5% CO2, 37°C incubator for 1 hour. The fluorescence signal was monitored at Ex/Em = 540/570 nm (cut off = 550 nm) with bottom read mode.

  • Hela cells stained with the Cellular Reactive Oxygen Species Detection Assay Kit (Orange Fluorescence) (ab186028)
    Hela cells stained with the Cellular Reactive Oxygen Species Detection Assay Kit (Orange Fluorescence) (ab186028)

    Hela cells stained with the Cellular Reactive Oxygen Species Detection Assay Kit (Orange Fluorescence) (ab186028) in a black wall/clear bottom 96-well plate. A: Untreated control cells. B: Cells treated with 100 µM TBHP for 30min before staining.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab186028? Please let us know so that we can cite the reference in this datasheet.

ab186028 has been referenced in 3 publications.

  • D'Onofrio N  et al. Phenolic Profiles of Red Wine Relate to Vascular Endothelial Benefits Mediated by SIRT1 and SIRT6. Int J Mol Sci 22:N/A (2021). PubMed: 34073604
  • Zhou X  et al. DPP4 Inhibitor Attenuates Severe Acute Pancreatitis-Associated Intestinal Inflammation via Nrf2 Signaling. Oxid Med Cell Longev 2019:6181754 (2019). PubMed: 31827684
  • Bilici K  et al. Indocyanine green loaded APTMS coated SPIONs for dual phototherapy of cancer. J Photochem Photobiol B 201:111648 (2019). PubMed: 31710924

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab186028.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.