Cellular ROS Assay Kit (Red) (ab186027)

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Overview

  • Product name

    Cellular ROS Assay Kit (Red)
    See all Oxidative Stress kits

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Cell-based (quantitative)

  • Assay time

    0h 60m

  • Product overview

    Cellular ROS Assay Kit (Red) ab186027 uses a ROS sensor to quantify ROS (Reactive Oxygen Species) in live cells. The red dye used in the ROS assay protocol is cell-permeable and generates red fluorescence when it reacts with ROS. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments.


    ab186027 provides an ultrasensitive fluorometric one-step ROS assay. The ROS assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 520/605 nm and can be used to either quantify ROS levels in cells or to screen ROS inhibitors.

  • Notes

    Previously called Cellular Reactive Oxygen Species Detection Assay Kit (Red Fluorescence).

    Reactive oxygen species (ROS) are natural byproducts of the normal metabolism of oxygen and play important roles in cell signaling. However, during oxidative stress-related states, ROS levels can increase dramatically. The accumulation of ROS results in significant damage to cell structures. The role of oxidative stress in cardiovascular disease, diabetes, osteoporosis, stroke, inflammatory diseases, a number of neurodegenerative diseases and cancer has been well established. The ROS measurement will help to determine how oxidative stress modulates varied intracellular pathways.

    Related products

    Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

    To measure reactive oxygen species within cells, we recommend DCFDA / H2DCFDA - Cellular ROS Assay Kit ab113851. Alternative ROS assays are available in orange (ab186028), red (ab186027), and deep red (ab186029).  ab238535 is used to measure ROS in biofluids, culture supernatants and cell lysates. 

    For assays designed to differentiate ROS, superoxides, and reactive nitrogen species: to assay ROS and superoxides use ab139476; to assay ROS, superoxides, and reactive nitrogen species use ab139473; to assay superoxides use ab219943.

  • Platform

    Microplate reader, Fluorescence microscope

Properties

Associated products

    Images

    • Functional Studies - Cellular Reactive Oxygen Species Detection Assay Kit (ab186027)
      Functional Studies - Cellular Reactive Oxygen Species Detection Assay Kit (ab186027)

      Bar graph showing relative fluorescence of Reactive Oxygen Species induced in various quantities of Hela cells at various concentrations of H2O2 after 30 min. exposure time.

    • Functional Studies - Cellular Reactive Oxygen Species Detection Assay Kit (ab186027)
      Functional Studies - Cellular Reactive Oxygen Species Detection Assay Kit (ab186027)

      Bar graph showing relative fluorescence of Reactive Oxygen Species induced in various quantities of Hela cells at various concentrations of H2O2 after 120 min. exposure time.

    References (3)

    Publishing research using ab186027? Please let us know so that we can cite the reference in this datasheet.

    ab186027 has been referenced in 3 publications.

    • Li M  et al. Mitochondrial Fusion Via OPA1 and MFN1 Supports Liver Tumor Cell Metabolism and Growth. Cells 9:N/A (2020). PubMed: 31947947
    • Igwebuike C  et al. Cross organelle stress response disruption promotes gentamicin-induced proteotoxicity. Cell Death Dis 11:217 (2020). PubMed: 32245975
    • Yang H  et al. SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 15:1539-1557 (2019). PubMed: 30871407

    Customer reviews and Q&As

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    Question

    1. Between the cell staining and treatment steps, if I add a wash step to remove the stain will the dye still be active in the cells for my treatment and subsequent measurement?
    2. Does the assay buffer contain anything that will maintain viability? Instead of the assay buffer, can I use my phenol red free media?
    Thank you, I look forward to your reply

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    Answer

    1. Between the cell staining and treatment steps, if I add a wash step to remove the stain will the dye still be active in the cells for my treatment and subsequent measurement?__it should be OK, but might decrease the signal a bit. You might try both situations (with and without wash) due to the fact that the dye can get in and out cells freely if there is no ROS in the cells.

    2. Does the assay buffer contain anything that will maintain viability?__It is HH buffer. Instead of the assay buffer, can I use my phenol red free media?__Yes, it should be OK.

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