Key features and details
- Assay type: Cell-based
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Product nameCellular ROS/RNS Assay Kit
See all Oxidative Stress kits
Sample typeAdherent cells, Suspension cells
Species reactivityReacts with: Mammals, Other species
Cellular ROS/RNS Assay Kit (ab139473) is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three fluorescent dye reagents: NO Detection Reagent (Red), Oxidative Stress Detection Reagent (Green) for total ROS detection, and Superoxide Detection Reagent (Orange). It also includes positive controls, pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite.
Storage instructionsPlease refer to protocols.
Components 200 tests 10 X Wash Buffer 1 x 15ml NO Detection Reagent (Red) 1 x 60µl NO Inducer (L-Arginine) 1 x 100µl NO Scavenger (c-PTIO) 1 x 400nmole Oxidative Stress Detection Reagent (Green) 1 x 300nmole ROS Inducer (Pyocyanin 1 µmole) 1 vial ROS Inhibitor (N-acetyl-L-cysteine) 2 x 10mg Superoxide Detection Reagent (Orange) 1 x 300nmole
ab139473 has been referenced in 3 publications.
- Kochanowsky JA et al. ROP16-Mediated Activation of STAT6 Suppresses Host Cell Reactive Oxygen Species Production, Facilitating Type III Toxoplasma gondii Growth and Survival. mBio 12:N/A (2021). PubMed: 33653884
- Liu B et al. JS-K, a nitric oxide donor, induces autophagy as a complementary mechanism inhibiting ovarian cancer. BMC Cancer 19:645 (2019). PubMed: 31262254
- Li H et al. Evaluation of the protective potential of brain microvascular endothelial cell autophagy on blood-brain barrier integrity during experimental cerebral ischemia-reperfusion injury. Transl Stroke Res 5:618-26 (2014). PubMed: 25070048