Overview

  • Product name

    Cellular Superoxide Detection Assay Kit
  • Sample type

    Adherent cells, Suspension cells
  • Assay type

    Cell-based
  • Product overview

    Cellular Superoxide Detection Assay Kit (ab139477) is designed to directly monitor real time superoxide production in live cells using fluorescence microscopy and/or flow cytometry. A major component of the kit, Superoxide Detection Reagent (Orange), is a cell-permeable probe that reacts specifically with superoxide, generating an orange fluorescent product. The kit is not designed to detect reactive peroxide, hydroxyl, peroxynitrite, chlorine or bromine species, as the fluorescent probe included is relatively insensitive to these analytes. Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with standard orange (e.g. 550/620 nm) or red (e.g. 650/670 nm) fluorescent cubes, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser.


    The Cellular Superoxide Detection Assay kit contains sufficient reagents for at least 200 microscopy assays or 50 flow cytometry assays using live cells (adherent or in suspension).

  • Notes

    Related products

    Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

  • Platform

    Flow cytometer, Fluorescence microscope

Properties

Images

  • Cellular Superoxide Detection Assay Kit (ab139477) Jurkat cells were induced with 100 µM pyocyanin (general ROS inducer, panel A), or 200 µM antimycin A (superoxide inducer, panel B), stained with Superoxide Detection Reagent (Orange) and analyzed using flow cytometry. Untreated cells (tinted profile) were used as a control. Cell debris were ungated. The numbers within the inserts reflect the mean orange fluorescence of the cells treated and control samples.

Protocols

References

ab139477 has not yet been referenced specifically in any publications.

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