Overview

  • Product name

  • Description

    Rabbit polyclonal to CELSR1
  • Host species

    Rabbit
  • Specificity

    ab196600 detects endogenous levels of total CELSR1 protein.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human CELSR1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NYQ6

  • Positive control

    • MCF7 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab196600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.

Target

  • Relevance

    CELSR1 is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor like repeats and two laminin A G type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact mediated communication, with cadherin domains acting as homophilic binding regions and the EGF like domains involved in cell adhesion and receptor ligand interactions. This particular member is a developmentally regulated, neural specific gene which plays an unspecified role in early embryogenesis.ESTs have been isolated from B-cell/lung/testis, breast, brain, colon, embryo, head/neck, heart, heart/melanocyte/uterus, kidney, lung, lymph node, placenta, and tonsil libraries.
  • Cellular localization

    Integral membrane protein
  • Database links

  • Alternative names

    • Cadherin EGF LAG seven pass G type receptor antibody
    • Cadherin EGF LAG seven pass G type receptor 1 antibody
    • CDHF 9 antibody
    • CDHF9 antibody
    • CELS R1 antibody
    • CELSR 1 antibody
    • DKFZp434P0729 antibody
    • Flamingo homolog 2 antibody
    • Flamingo homolog antibody
    • Flamingo homolog Drosophila antibody
    • FMI 2 antibody
    • FMI2 antibody
    • HFMI 2 antibody
    • HFMI2 antibody
    • ME 2 antibody
    • ME2 antibody
    • OTTHUMP00000028852 antibody
    • Protocadherin flamingo 2 antibody
    see all

Images

  • Immunofluorescence analysis of MCF-7 cells, labeling CELSR1 with ab196600 at 1/100 dilution.

References

ab196600 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Sample
Mouse Tissue sections (Adult Female Reproductive Tissue - Oviduct)
Specification
Adult Female Reproductive Tissue - Oviduct
Permeabilization
Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 28 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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