Key features and details
- Rabbit polyclonal to CELSR1
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-CELSR1 antibody
See all CELSR1 primary antibodies
DescriptionRabbit polyclonal to CELSR1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CELSR1 aa 2775-2825. The exact sequence is proprietary. (NP_055061.1).
Database link: Q9NYQ6
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225889 was affinity purified using an epitope specific to TRAF2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 329 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceCELSR1 is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor like repeats and two laminin A G type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact mediated communication, with cadherin domains acting as homophilic binding regions and the EGF like domains involved in cell adhesion and receptor ligand interactions. This particular member is a developmentally regulated, neural specific gene which plays an unspecified role in early embryogenesis.ESTs have been isolated from B-cell/lung/testis, breast, brain, colon, embryo, head/neck, heart, heart/melanocyte/uterus, kidney, lung, lymph node, placenta, and tonsil libraries.
Cellular localizationIntegral membrane protein
- Cadherin EGF LAG seven pass G type receptor antibody
- Cadherin EGF LAG seven pass G type receptor 1 antibody
- CDHF 9 antibody
All lanes : Anti-CELSR1 antibody (ab225889) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 329 kDa
Exposure time: 3 minutes
Lysates prepared using NETN buffer.
CELSR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225889 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225889 at 0.4 µg/ml.
Lane 1: ab225889 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225889 has not yet been referenced specifically in any publications.