Overview

  • Product name

  • Description

    Rabbit polyclonal to CELSR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CELSR1 aa 2775-2825. The exact sequence is proprietary. (NP_055061.1).
    Database link: Q9NYQ6

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 329 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    CELSR1 is a member of the flamingo subfamily, part of the cadherin superfamily. The flamingo subfamily consists of nonclassic type cadherins; a subpopulation that does not interact with catenins. The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor like repeats and two laminin A G type repeats in their ectodomain. They also have seven transmembrane domains, a characteristic unique to this subfamily. It is postulated that these proteins are receptors involved in contact mediated communication, with cadherin domains acting as homophilic binding regions and the EGF like domains involved in cell adhesion and receptor ligand interactions. This particular member is a developmentally regulated, neural specific gene which plays an unspecified role in early embryogenesis.ESTs have been isolated from B-cell/lung/testis, breast, brain, colon, embryo, head/neck, heart, heart/melanocyte/uterus, kidney, lung, lymph node, placenta, and tonsil libraries.
  • Cellular localization

    Integral membrane protein
  • Database links

  • Alternative names

    • Cadherin EGF LAG seven pass G type receptor antibody
    • Cadherin EGF LAG seven pass G type receptor 1 antibody
    • CDHF 9 antibody
    • CDHF9 antibody
    • CELS R1 antibody
    • CELSR 1 antibody
    • DKFZp434P0729 antibody
    • Flamingo homolog 2 antibody
    • Flamingo homolog antibody
    • Flamingo homolog Drosophila antibody
    • FMI 2 antibody
    • FMI2 antibody
    • HFMI 2 antibody
    • HFMI2 antibody
    • ME 2 antibody
    • ME2 antibody
    • OTTHUMP00000028852 antibody
    • Protocadherin flamingo 2 antibody
    see all

Images

  • All lanes : Anti-CELSR1 antibody (ab225889) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 329 kDa


    Exposure time: 3 minutes


    Lysates prepared using NETN buffer.

  • CELSR1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225889 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225889 at 0.4 µg/ml.

    Lane 1: ab225889 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

References

ab225889 has not yet been referenced specifically in any publications.

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