For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    cemp1-antibody-ab134231.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Other Antibodies Other Antibodies
Share by email

Anti-CEMP1 antibody (ab134231)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (4)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-CEMP1 antibody (ab134231)
  • Western blot - Anti-CEMP1 antibody (ab134231)

Key features and details

  • Rabbit polyclonal to CEMP1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CEMP1 antibody
    See all CEMP1 primary antibodies
  • Description

    Rabbit polyclonal to CEMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 136-185 (RHFLNWALQQ REENSGRARR VPPVPRTAPV SKGEGSHPPQ NSNGEKVKTI) of Human CEMP1 (NP_001041677)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HeLa and HepG2 cell lysates.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa.

Target

  • Tissue specificity

    Detected in periodontal ligament, cementum, cementoblasts and cementoblastoma.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q6PRD7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 752014 Human
    • Omim: 611113 Human
    • SwissProt: Q6PRD7 Human
    • Unigene: 433499 Human
    • Unigene: 737156 Human
    • Unigene: 743305 Human
    • Alternative names

      • Cementoblastoma-derived protein 1 antibody
      • Cementum protein 1 antibody
      • Cementum protein 23 antibody
      • CEMP1 antibody
      • CEMP1_HUMAN antibody
      • CP-23 antibody
      • CP23 antibody
      see all

    Images

    • Western blot - Anti-CEMP1 antibody (ab134231)
      Western blot - Anti-CEMP1 antibody (ab134231)
      Anti-CEMP1 antibody (ab134231) at 1 µg/ml + HeLa cell lysate at 10 µg

      Predicted band size: 26 kDa



      Blocked in 5% milk.
    • Western blot - Anti-CEMP1 antibody (ab134231)
      Western blot - Anti-CEMP1 antibody (ab134231)
      Anti-CEMP1 antibody (ab134231) at 1 µg/ml + HepG2 cell lysate at 10 µg

      Predicted band size: 26 kDa

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab134231? Please let us know so that we can cite the reference in this datasheet.

    ab134231 has been referenced in 4 publications.

    • Li Q  et al. EZH2 reduction is an essential mechanoresponse for the maintenance of super-enhancer polarization against compressive stress in human periodontal ligament stem cells. Cell Death Dis 11:757 (2020). PubMed: 32934212
    • Hong HH  et al. Calcitriol exerts a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells. Am J Transl Res 11:2304-2316 (2019). PubMed: 31105837
    • Li S  et al. The impact of Wnt signalling and hypoxia on osteogenic and cementogenic differentiation in human periodontal ligament cells. Mol Med Rep 14:4975-4982 (2016). WB ; Human . PubMed: 27840938
    • Lee CH  et al. Three-dimensional printed multiphase scaffolds for regeneration of periodontium complex. Tissue Eng Part A 20:1342-51 (2014). PubMed: 24295512

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CEMP1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tooth, Alveolar bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Tooth, Alveolar bone
    Blocking step
    Blocking One Histo as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 31 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CEMP1 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (Tooth)
    Specification
    Tooth
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 10 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CEMP1 antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: proteinase K
    Sample
    Human Tissue sections (tissues made by human PDL cells in mice)
    Specification
    tissues made by human PDL cells in mice
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 06 2014

    Question

    Publication IHC par Emoto.
    Kits EpiSeeker
    Anti-CEMP1 souris

    Read More

    Abcam community

    Verified customer

    Asked on Jul 03 2012

    Answer


    1) La publication que j'ai mentionnée à propos du démasquage antigène en IHC est la suivante: Emoto et al., J Histochem Cytochem. 2005 53 (11) : 1311-21.
    2) Les détails concernant les kits EpiSeeker sont disponibles via le lien suivant: www.abcam.com/episeeker.
    3) Nous avons 2 anti-CEMP1, le ab134231 et le ab121447, tous 2 testés chez l'homme. Je n'ai malheureusement pas trouvé la séquence de la CEMP1 murine et je n'ai donc pas pu déterminer l'homologie de séquence entre les immunogènes et la protéine cible.
    Si vous souhaitez tester un de ces 2 anticorps anti-CEMP1 chez la souris, je pourrai vous offrir une offre de test :
    1. M'informer que vous souhaitez tester ab134231 ou ab121447 chez la souris et je vous enverrai un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,
    2. Acheter ab134231 ou ab121447,
    3. Nous envoyer vos résultats (positifs ou négatifs) obtenus chez la souris sous forme d'Abreview (www.abcam.com/abreviews). Ceci activera le code.
    4. Commander gratuitement un anticorps primaire Abcam de votre choix en utilisant le code promotionnel.

    Read More

    Abcam Scientific Support

    Answered on Jul 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.