Anti-CEMP1 antibody (ab134231)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-CEMP1 antibody
See all CEMP1 primary antibodies -
Description
Rabbit polyclonal to CEMP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region within C terminal amino acids 136-185 (RHFLNWALQQ REENSGRARR VPPVPRTAPV SKGEGSHPPQ NSNGEKVKTI) of Human CEMP1 (NP_001041677)
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Positive control
- HeLa cell lysate This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use 10µg for 10 cells. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 26 kDa. |
Target
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Tissue specificity
Detected in periodontal ligament, cementum, cementoblasts and cementoblastoma. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 752014 Human
- Omim: 611113 Human
- SwissProt: Q6PRD7 Human
- Unigene: 433499 Human
- Unigene: 737156 Human
- Unigene: 743305 Human
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Alternative names
- Cementoblastoma-derived protein 1 antibody
- Cementum protein 1 antibody
- Cementum protein 23 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CEMP1 antibody (ab134231)ICC/IF image of ab134231 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab134231 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-CEMP1 antibody (ab134231)Anti-CEMP1 antibody (ab134231) at 1 µg/ml + HeLa cell lysate at 10 µg
Predicted band size: 26 kDa
Blocked in 5% milk.
Datasheets and documents
References
This product has been referenced in:
- Hong HH et al. Calcitriol exerts a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells. Am J Transl Res 11:2304-2316 (2019). Read more (PubMed: 31105837) »
- Li S et al. The impact of Wnt signalling and hypoxia on osteogenic and cementogenic differentiation in human periodontal ligament cells. Mol Med Rep 14:4975-4982 (2016). WB ; Human . Read more (PubMed: 27840938) »
