Key features and details
- Rabbit polyclonal to Cenexin1/ODF2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cenexin1/ODF2 antibody
See all Cenexin1/ODF2 primary antibodies
DescriptionRabbit polyclonal to Cenexin1/ODF2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Bat
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human Cenexin1/ODF2 (NP_702911.1).
- HeLa whole cell lysate 293T whole cell lysate
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. The product can be stored for up to 12 months.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125192 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 95 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionSeems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
Tissue specificityTestis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
Sequence similaritiesBelongs to the ODF2 family.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
- Information by UniProt
- 84 kDa outer dense fiber protein antibody
- Cancer/testis antigen 134 antibody
- Cenexin 1 antibody
All lanes : Anti-Cenexin1/ODF2 antibody (ab125192) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 95 kDa
Exposure time: 3 minutes
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab125192 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in lane
Western Blot: Cenexin1/ODF2 antibody (ab125192) at 1 µg/ml
developed using the ECL technique
Exposure time : 30 seconds
ab125192 has not yet been referenced specifically in any publications.