Overview

  • Product name

    Anti-Cenexin1/ODF2 antibody [EPR13561]
    See all Cenexin1/ODF2 primary antibodies
  • Description

    Rabbit monoclonal [EPR13561] to Cenexin1/ODF2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cenexin1/ODF2 aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: Q5BJF6

  • Positive control

    • Jurkat, Hela, HepG2, human testis lysates, Hela cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 95 kDa.
Flow Cyt 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
  • Tissue specificity

    Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
  • Sequence similarities

    Belongs to the ODF2 family.
  • Post-translational
    modifications

    Tyrosine phosphorylated.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
  • Information by UniProt
  • Database links

  • Alternative names

    • 84 kDa outer dense fiber protein antibody
    • Cancer/testis antigen 134 antibody
    • Cenexin 1 antibody
    • Cenexin antibody
    • Cenexin1 variant 1 antibody
    • CT134 antibody
    • FLJ44866 antibody
    • KKT 4 antibody
    • KKT4 antibody
    • MGC111096 antibody
    • MGC9034 antibody
    • ODF 2 antibody
    • ODF 84 antibody
    • odf2 antibody
    • ODF2/1 antibody
    • ODF2/2 antibody
    • ODF84 antibody
    • ODFP2_HUMAN antibody
    • OTTHUMP00000022274 antibody
    • OTTHUMP00000022275 antibody
    • Outer dense fiber of sperm tail 2 antibody
    • Outer dense fiber of sperm tails 2 antibody
    • Outer dense fiber of sperm tails protein 2 antibody
    • Outer dense fiber of sperm tails, 84 kD antibody
    • Outer dense fiber protein 2 antibody
    • Sperm outer dense fiber major protein 2 antibody
    • Sperm tail structural protein antibody
    • Testis specific autoantigen antibody
    see all

Images

  • Flow cytometric analysis of paraformaldehyde fixed Hela cells labeling Cenexin1/ODF2 with ab186412 at 1/250 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution

  • Anti-Cenexin1/ODF2 antibody [EPR13561] (ab186412) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 95 kDa

  • All lanes : Anti-Cenexin1/ODF2 antibody [EPR13561] (ab186412) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 95 kDa

References

ab186412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X-100
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Jan 14 2019

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