Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13561] to Cenexin1/ODF2
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-Cenexin1/ODF2 antibody [EPR13561]
See all Cenexin1/ODF2 primary antibodies
DescriptionRabbit monoclonal [EPR13561] to Cenexin1/ODF2
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Cenexin1/ODF2 aa 800 to the C-terminus. The exact sequence is proprietary.
Database link: Q5BJF6
- Jurkat, Hela, HepG2, human testis lysates, Hela cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab186412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 95 kDa.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionSeems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
Tissue specificityTestis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
Sequence similaritiesBelongs to the ODF2 family.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > spindle pole. Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
- Information by UniProt
- 84 kDa outer dense fiber protein antibody
- Cancer/testis antigen 134 antibody
- Cenexin 1 antibody
Flow cytometric analysis of paraformaldehyde fixed Hela cells labeling Cenexin1/ODF2 with ab186412 at 1/250 dilution and Goat anti-rabbit IgG (FITC) at 1/150 dilution
Anti-Cenexin1/ODF2 antibody [EPR13561] (ab186412) at 1/1000 dilution + Human testis lysate at 10 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 95 kDa
All lanes : Anti-Cenexin1/ODF2 antibody [EPR13561] (ab186412) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Hela cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 95 kDa
ab186412 has not yet been referenced specifically in any publications.