Anti-CENH3 antibody (ab72001)

Submit a review Q&A (2)References (3)

Key features and details

  • Rabbit polyclonal to CENH3
  • Reacts with: Arabidopsis thaliana
  • Isotype: IgG

Overview

  • Product name

    Anti-CENH3 antibody

  • Description

    Rabbit polyclonal to CENH3

  • Host species

    Rabbit

  • Species reactivity

    Reacts with: Arabidopsis thaliana

  • Immunogen

    Synthetic peptide corresponding to Arabidopsis thaliana CENH3 (N terminal).

  • General notes

    Previously labelled as HTR12. 

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Constituent: Whole serum
  • Concentration information loading...

  • Purity

    Whole antiserum

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Target

  • Relevance

    HTR12 is a histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. It is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation and may serve as an epigenetic mark that propagates centromere identity through replication and cell division.

  • Cellular localization

    Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.

  • Database links

    • Alternative names

      • CENH3 antibody
      • Centromeric histone CENH3 antibody
      • F6F3.17 antibody
      • F6F3_17 antibody
      • Histone H3 like centromeric protein HTR12 antibody
      • HTR 12 antibody
      • HTR12 antibody
      see all

    References (3)

    Publishing research using ab72001? Please let us know so that we can cite the reference in this datasheet.

    ab72001 has been referenced in 3 publications.

    • Xu RY  et al. The Arabidopsis anaphase-promoting complex/cyclosome subunit 8 is required for male meiosis. New Phytol 224:229-241 (2019). PubMed: 31230348
    • Karimi-Ashtiyani R  et al. Point mutation impairs centromeric CENH3 loading and induces haploid plants. Proc Natl Acad Sci U S A 112:11211-6 (2015). WB . PubMed: 26294252
    • Mérai Z  et al. The AAA-ATPase molecular chaperone Cdc48/p97 disassembles sumoylated centromeres, decondenses heterochromatin, and activates ribosomal RNA genes. Proc Natl Acad Sci U S A 111:16166-71 (2014). PubMed: 25344531

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question


    I would like to know what epitope was used for its production and if possible any references and its specificity towards CENH3 compared to other histones.

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    Answer


    Unfortunately the immunogen sequence is considered proprietary (commercially sensitive and confidential) and I can not disclose it at this time. However, I can confirm that the immunogen is a peptide of 20 amino acids located between 1 and 40 amino acids of the A. thaliana HTR12.

    There are currently no references for ab72001. Regarding cross reactivity, the lab have confirmed that ab72001 shows no cross-reactivity with other histones.

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    Question

    I am interested in ab72001 product, but I need the information regarding immunogen sequence used to make the antibody, to design my experiment in a better way..
    and expecting your reply soon
    thanks

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    Answer

    Thank you very much for contacting us with your question.

    Unfortunately the immunogen sequence is considered commercially sensitive and we can not disclose it at this time. I can onlyconfirm that it is taken from the first 100 N-terminal amino acids.

    If you'd like to describe the experiment that you would like to design, I may be able to help you further. Could you tell me what kind of assay you're planningto run and what kind of samples you will use?

    I look forward to hearing from you.I am sorry thatI do not have the information that you're looking for, but if you have further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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