Recombinant Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP800Y] to CENPA - Centromere Marker
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CENPA antibody [EP800Y] - Centromere Marker
See all CENPA primary antibodies -
Description
Rabbit monoclonal [EP800Y] to CENPA - Centromere Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human colon adenocarcinoma, lung carcinoma and prostate carcinoma tissue. Human skin and colon tissue. Human lymphoma tissue. WB: HeLa, A375 and HCT-116 whole cell lysate. Human melanoma. Human placenta lysate.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP800Y -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/20000. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. -
Sequence similarities
Belongs to the histone H3 family. -
Domain
The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containing CENPA and is necessary and sufficient to mediate the localization into centromeres. -
Post-translational
modificationsUbiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway.
Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height. - Information by UniProt
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Database links
- Entrez Gene: 1058 Human
- Omim: 117139 Human
- SwissProt: P49450 Human
- Unigene: 1594 Human
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Alternative names
- CENP A antibody
- CENP-A antibody
- cenpa antibody
see all
Images
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Immunohistochemical analysis of formalin fixed, paraffin embedded colonic adenocarcinoma tissue section labelling CENPA with unpurified ab45694.
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All lanes : Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/1000 dilution (purified)
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : Human melanoma
Lane 3 : A375 (human malignant melanoma) whole cell lysate
Lane 4 : HCT-116 (human colorectal carcinoma) whole cell lysate
Lane 5 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Blocking and diluting buffer 5% NFDM/TBST -
Anti-CENPA antibody [EP800Y] - Centromere Marker (ab45694) at 1/20000 dilution (unpurified) + HeLa cell lysate (10ug/lane)
Predicted band size: 16 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded human colon tissue section labelling CENPA with purified ab45694 at dilution of 1/250. The secondary antibody used was HRP-conjugated Goat Anti-Rabbit IgG H&L (ab97051) at dilution of 1/500. The sample was counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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IHC image of ab45694 staining CENPA in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab45694, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunohistochemical analysis of formalin fixed, paraffin embedded colonic adenocarcinoma tissue section labelling CENPA with unpurified ab45694.
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Immunohistochemical analysis of formalin fixed, paraffin embedded Lymphoma tissue section labelling CENPA with unpurified ab45694.
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Immunohistochemical analysis of formalin fixed, paraffin embedded prostatic carcinoma tissue section labelling CENPA with unpurified ab45694.
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Immunohistochemical analysis of formalin fixed, paraffin embedded lung carcinoma tissue section labelling CENPA with unpurified ab45694.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab45694 has been referenced in 5 publications.
- Wei J et al. Geranylgeranylation signaling promotes breast cancer cell mitosis via the YAP-activated transcription of kinetochore/centromere genes. Am J Cancer Res 12:1143-1155 (2022). PubMed: 35411228
- Celikkan FT et al. PFA is superior to glyoxal in preserving oocyte, embryo, and stem cell proteins evidenced by super-resolution microscopical surveys of epitopes. J Assist Reprod Genet 37:369-384 (2020). PubMed: 31930433
- Tang H et al. miR-505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT-NF?B pathway in NSCLC cells. Int J Mol Med 43:1203-1216 (2019). PubMed: 30628663
- Cheng Z et al. circTP63 functions as a ceRNA to promote lung squamous cell carcinoma progression by upregulating FOXM1. Nat Commun 10:3200 (2019). PubMed: 31324812
- Taylor DA et al. Building a Total Bioartificial Heart: Harnessing Nature to Overcome the Current Hurdles. Artif Organs N/A:N/A (2018). PubMed: 30044011