Product nameAnti-CENPB antibody
See all CENPB primary antibodies
DescriptionRabbit polyclonal to CENPB
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Hamster
- This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Testis (Mouse) Tissue Lysate - normal tissue Ovary (Mouse) Tissue Lysate - normal tissue PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab25734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInteracts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.
Sequence similaritiesContains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain.
Cellular localizationNucleus. Chromosome > centromere.
- Information by UniProt
- CENP B antibody
- CENP-B antibody
- CENPB antibody
All lanes : Anti-CENPB antibody (ab25734) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Jurkat whole cell lysate (ab7899)
Lane 3 :
A431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 74 kDa (possible cross reactivity), 75 kDa (possible cross reactivity)
4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CENPB (red) using ab25734 at 1/200 dilution in ICC/IF.
The nuclear counterstain is DAPI (blue). Alpha-Tubulin is stained with an mouse monoclonal anti-alpha Tubulin antibody (green).
Image courtesy of Human Protein Atlas. ab25734 staining CENPB in Human skin, showing nuclear staining in the epidermis and dermal layer. Paraffin embedded human skin tissue was incubated with ab25734(1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab25734 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
ab25734 (1/1000) staining CENPB in HeLa cells (green). Cells were fixed with methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
ICC/IF image of ab25734 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab25734, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293 cells.
This product has been referenced in:
- Thakur J & Henikoff S Unexpected conformational variations of the human centromeric chromatin complex. Genes Dev 32:20-25 (2018). Read more (PubMed: 29386331) »
- Tiwari A et al. 53BP1 can limit sister-chromatid rupture and rearrangements driven by a distinct ultrafine DNA bridging-breakage process. Nat Commun 9:677 (2018). Read more (PubMed: 29445165) »