Anti-CENPB antibody (ab84489)
Key features and details
- Rabbit polyclonal to CENPB
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CENPB antibody
See all CENPB primary antibodies -
Description
Rabbit polyclonal to CENPB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region between residues 540 and the C-terminus (residue 599) of human CENPB using the numbering given in entry SwissProt entry P07199
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Positive control
- HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to CENP-B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. -
Sequence similarities
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain. -
Cellular localization
Nucleus. Chromosome > centromere. - Information by UniProt
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Database links
- Entrez Gene: 1059 Human
- Omim: 117140 Human
- SwissProt: P07199 Human
- Unigene: 516855 Human
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Alternative names
- CENP B antibody
- CENP-B antibody
- CENPB antibody
see all
Images
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ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.
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ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.
Protocols
Datasheets and documents
References (1)
ab84489 has been referenced in 1 publication.
- Anton T et al. Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system. Nucleus 5:163-72 (0). IF . PubMed: 24637835