Anti-CENPB antibody (ab84489)

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Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)
  • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)

Key features and details

  • Rabbit polyclonal to CENPB
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CENPB antibody
    See all CENPB primary antibodies

  • Description

    Rabbit polyclonal to CENPB

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to a region between residues 540 and the C-terminus (residue 599) of human CENPB using the numbering given in entry SwissProt entry P07199

  • Positive control

    • HeLa cells

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Purified using an epitope specific to CENP-B immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/500.
    Permeabilization with Triton-X 100 is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes.

    • Sequence similarities

      Contains 1 HTH CENPB-type DNA-binding domain.
      Contains 1 HTH psq-type DNA-binding domain.

    • Cellular localization

      Nucleus. Chromosome > centromere.
    • Information by UniProt

    • Database links

      • Alternative names

        • CENP B antibody
        • CENP-B antibody
        • CENPB antibody
        • CENPB_HUMAN antibody
        • centromere autoantigen B antibody
        • Centromere protein B antibody
        • Centromere protein B, 80kDa antibody
        • Major centromere autoantigen B antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)
        Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)
        ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.
      • Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)
        Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)
        ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.

      References (1)

      Publishing research using ab84489? Please let us know so that we can cite the reference in this datasheet.

      ab84489 has been referenced in 1 publication.

      • Anton T  et al. Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system. Nucleus 5:163-72 (0). IF . PubMed: 24637835

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