Anti-CENPB antibody (ab84489)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CENPB antibody
See all CENPB primary antibodies -
Description
Rabbit polyclonal to CENPB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to a region between residues 540 and the C-terminus (residue 599) of human CENPB using the numbering given in entry SwissProt entry P07199
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Positive control
- HeLa cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified using an epitope specific to CENP-B immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab84489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Application notes
ICC/IF: 1/100 - 1/500.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Permeabilization with Triton-X 100 is recommended.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. -
Sequence similarities
Contains 1 HTH CENPB-type DNA-binding domain.
Contains 1 HTH psq-type DNA-binding domain. -
Cellular localization
Nucleus. Chromosome > centromere. - Information by UniProt
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Database links
- Entrez Gene: 1059 Human
- Omim: 117140 Human
- SwissProt: P07199 Human
- Unigene: 516855 Human
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Alternative names
- CENP B antibody
- CENP-B antibody
- CENPB antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100. -
Immunocytochemistry/ Immunofluorescence - Anti-CENPB antibody (ab84489)ICC analysis of NBF-fixed asynchronous HeLa cells using ab84489 at a dilution of 1:250. Detection: Anti-rabbit IgG-FITC-conjugated antibody used at a dilution of 1/100.
Datasheets and documents
References
This product has been referenced in:
- Anton T et al. Visualization of specific DNA sequences in living mouse embryonic stem cells with a programmable fluorescent CRISPR/Cas system. Nucleus 5:163-72 (0). IF . Read more (PubMed: 24637835) »
