Product nameAnti-CENPC antibody
See all CENPC primary antibodies
DescriptionRabbit polyclonal to CENPC
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate and Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab33034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 107 kDa).|
FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.
Sequence similaritiesBelongs to the CENPC family.
DomainThe MIF2 homology domain II targets centromeres and binds the alpha satellite DNA in vivo. The MIF2 homology domain III can induce CENPC dimerization/oligomerization.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
- Information by UniProt
- CENP-C 1 antibody
- CENP-C antibody
- CENPC_HUMAN antibody
All lanes : Anti-CENPC antibody (ab33034) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 :
Jurkat whole cell lysate (ab7899) at 20 µg
All lanes : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 107 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
The observed band size of 140 kDa is consistent with the banding patterns observed when using other commercial antibodies to this target.
ICC/IF image of ab33034 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab33034, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
ab33034 (1/200) staining CENPC in HeLa cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.
This product has been referenced in:
- Thakur J & Henikoff S Unexpected conformational variations of the human centromeric chromatin complex. Genes Dev 32:20-25 (2018). Read more (PubMed: 29386331) »
- Eliezer Y et al. Interplay between the DNA damage proteins MDC1 and ATM in the regulation of the spindle assembly checkpoint. J Biol Chem 289:8182-93 (2014). WB . Read more (PubMed: 24509855) »