Product nameAnti-CENPE antibody
See all CENPE primary antibodies
DescriptionRabbit polyclonal to CENPE
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human CENPE aa 2050-2100. The exact sequence is proprietary. NP_001804.2
Database link: Q02224
- IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
Our Abpromise guarantee covers the use of ab264251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
RelevanceCENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase. In contrast to other centromere proteins, CENPE is not detected at centromeres during interphase, and first appears at the centromere region of chromosomes during prometaphase. CENPE function is required for the transition from metaphase to anaphase. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation.
Cellular localizationAssociates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
- CENP E antibody
- Centromere associated protein E antibody
- Centromere autoantigen E (312kD) antibody
CENPE was immunoprecipitated from 1mg of HeLa whole cell lysates using ab264251 at 3μg/mg. Western blot was performed on the immunprecipitates using a different CENPE antibody at 1μg/ml.
Lane 1: ab264251 IP in HeLa whole cell lysate.
lane 2: Control IgG IP in HeLa whole cell lysate.
Detected with chemilimuniscence with exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264251 has not yet been referenced specifically in any publications.