Overview

  • Product name

    Anti-CENPE antibody [EPR4542(2)]
    See all CENPE primary antibodies
  • Description

    Rabbit monoclonal [EPR4542(2)] to CENPE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to an internal sequence within amino acids 10-50 of Human CENPE (UniProt Q02224).

  • Positive control

    • HeLa treated with aphidicolin and Nocodazole, HepG2 and Jurkat cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 312 kDa.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Relevance

      CENPE is a 250-300 kDa human centromere-associated kinesin-like motor protein that accumulates in G2 phase. In contrast to other centromere proteins, CENPE is not detected at centromeres during interphase, and first appears at the centromere region of chromosomes during prometaphase. CENPE function is required for the transition from metaphase to anaphase. CENPE is probably one of the motors responsible for mammalian chromosome movement and/or spindle elongation.
    • Cellular localization

      Associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
    • Database links

    • Alternative names

      • CENP E antibody
      • Centromere associated protein E antibody
      • Centromere autoantigen E (312kD) antibody
      • Centromere autoantigen E antibody
      • Centromere protein E 312kDa antibody
      • Centromere protein E antibody
      • KIF10 antibody
      • Kinesin family member 10 antibody
      • Kinesin related protein antibody
      • Kinesin related protein CENPE antibody
      • PPP1R61 antibody
      • Protein phosphatase 1, regulatory subunit 61 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CENPE knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HepG2 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab133583 observed at 310 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133583 was shown to specifically react with CENPE when CENPE knockout samples were used. Wild-type and CENPE knockout samples were subjected to SDS-PAGE. ab133583 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • ab133583 staining CENPE in the Hela cell line from Human cervix by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. Ab150081 (1/200) was used as the secondary antibody.

      See Abreview

    • All lanes : Anti-CENPE antibody [EPR4542(2)] (ab133583) at 1/1000 dilution

      Lane 1 : HeLa treated with aphidicolin and Nocodazole cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 312 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 25 2017

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