Overview

  • Product name

  • Description

    Rabbit polyclonal to CENPH
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human CENPH aa 1-247.
    Sequence:

    MEEQPQMQDADEPADSGGEGRAGGPPQVAGAQAACSEDRMTLLLRLRAQT KQQLLEYKSMVDASEEKTPEQIMQEKQIEAKIEDLENEIEEVKVAFEIKK LALDRMRLSTALKKNLEKISRQSSVLMDNMKHLLELNKLIMKSQQESWDL EEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERIKIIRQ NLQMEIKITTVIQHVFQNLILGSKVNWAEDPALKEIVLQLEKNVDMM


    Database link: Q9H3R5

  • Positive control

    • WB: Mouse lung tissue lysate. IHC-P: Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab230193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/20 - 1/200.

Target

  • Function

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate.
  • Sequence similarities

    Belongs to the centromere protein H family.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Associates with active centromere-kinetochore complexes throughout the cell cycle. Colocalizes with inner kinetochore plate proteins CENPA and CENPC1 during both interphase and metaphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CENP H antibody
    • CENP-H antibody
    • CENPH antibody
    • CENPH_HUMAN antibody
    • Centromere protein H antibody
    • ICEN35 antibody
    • Interphase centromere complex protein 35 antibody
    • Kinetochore protein CENP H antibody
    • NNF1 antibody
    • NNF1, MIND kinetochore complex component, homolog antibody
    • PMF1 antibody
    see all

Images

  • Anti-CENPH antibody (ab230193) at 1/1000 dilution + Mouse lung tissue lysate

    Secondary
    Goat anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

  • Paraffin-embedded human tonsil tissue stained for CENPH with ab230193 at 1/100 dilution in immunohistochemical analysis.

References

ab230193 has not yet been referenced specifically in any publications.

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