Anti-CENPI antibody (ab168778)

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Overview

  • Product name

    Anti-CENPI antibody
    See all CENPI primary antibodies

  • Description

    Mouse polyclonal to CENPI

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein, corresponding to amino acids 1-522 of Human CENPI, Isoform 2 (AAH12462.1, UniProt ID: Q92674-2)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Lysate from 293T cells transfected with CENPI; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab168778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Relevance

    CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore.

  • Cellular localization

    Cytoplasmic and Nuclear

  • Database links

    • Alternative names

      • CENP I antibody
      • centromere protein I antibody
      • Follicle simulating hormone primary response protein antibody
      • FSH primary response 1 antibody
      • FSH primary response protein 1 antibody
      • FSH primary response, rat, homolog of, 1 antibody
      • FSHP1 antibody
      • FSHPRH1 antibody
      • interphase centromere complex protein 19 antibody
      • Leucine rich primary response gene 1 antibody
      • Leucine rich primary response protein 1 antibody
      • LRPR1 antibody
      • Mis6 antibody
      see all

    Images

    • Western blot - Anti-CENPI antibody (ab168778)
      Western blot - Anti-CENPI antibody (ab168778)
      All lanes : Anti-CENPI antibody (ab168778) at 1 µg/ml

      Lane 1 : CENPI-transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 15 µl per lane.

      Developed using the ECL technique.

      Predicted band size: 60 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (ab168778)
      Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (ab168778)
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CENPI using ab168778 at 10 µg/ml.

    References

    This product has been referenced in:

    • Hu L  et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment. Nucleic Acids Res 47:468-479 (2019). Read more (PubMed: 30407575) »
    See 1 Publication for this product

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