Anti-CENPI antibody (ab168778)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-CENPI antibody
See all CENPI primary antibodies -
DescriptionMouse polyclonal to CENPI
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Full length protein, corresponding to amino acids 1-522 of Human CENPI, Isoform 2 (AAH12462.1, UniProt ID: Q92674-2)
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Positive control
- Lysate from 293T cells transfected with CENPI; HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.20
Constituent: 100% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab168778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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RelevanceCENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore.
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 2491 Human
- Omim: 300065 Human
- SwissProt: Q92674 Human
- Unigene: 348920 Human
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Alternative names
- CENP I antibody
- centromere protein I antibody
- Follicle simulating hormone primary response protein antibody
see all
Images
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Western blot - Anti-CENPI antibody (ab168778)All lanes : Anti-CENPI antibody (ab168778) at 1 µg/ml
Lane 1 : CENPI-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 60 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (ab168778)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CENPI using ab168778 at 10 µg/ml.
Datasheets and documents
References
This product has been referenced in:
- Hu L et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment. Nucleic Acids Res 47:468-479 (2019). Read more (PubMed: 30407575) »
