Anti-CENPI antibody (ab168778)
Key features and details
- Mouse polyclonal to CENPI
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CENPI antibody
See all CENPI primary antibodies -
Description
Mouse polyclonal to CENPI -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Full length protein, corresponding to amino acids 1-522 of Human CENPI, Isoform 2 (AAH12462.1, UniProt ID: Q92674-2)
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Positive control
- Lysate from 293T cells transfected with CENPI; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa.
|
|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Relevance
CENPI is a constitutive protein of the knetochore that shares limited similarity with Mis6 of S. pombe. The kinetochore is a macromolecular complex located at the centromere of chromosomes and provides essential functions for chromosome segregration. CENPI is required for the localization of CENPF and the checkpoint proteins MAD1 and MAD2 to the kinetochore. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 2491 Human
- Omim: 300065 Human
- SwissProt: Q92674 Human
- Unigene: 348920 Human
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Alternative names
- CENP I antibody
- centromere protein I antibody
- Follicle simulating hormone primary response protein antibody
see all
Images
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Western blot - Anti-CENPI antibody (ab168778)All lanes : Anti-CENPI antibody (ab168778) at 1 µg/ml
Lane 1 : CENPI-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 60 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-CENPI antibody (ab168778)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling CENPI using ab168778 at 10 µg/ml.
Protocols
Datasheets and documents
References (1)
ab168778 has been referenced in 1 publication.
- Hu L et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment. Nucleic Acids Res 47:468-479 (2019). PubMed: 30407575
