Overview

  • Product name

    Anti-CENPM antibody
  • Description

    Rabbit polyclonal to CENPM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment corresponding to Human CENPM aa 6-81.
    Sequence:

    PLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLP SSVNRPRIDLIVFVVNLHSKYSLQNT


    Database link: Q9NSP4

  • Positive control

    • IHC-P: Human lymph node tissue. WB: HEK-293 cell lysate. ICC/IF: U-2 OS cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab243820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.4 µg/ml. Predicted molecular weight: 20 kDa.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100

Target

  • Relevance

    CENPM (Centromere protein M) is a component of the CENPA-NAC (nucleosome associated) complex, a complex that plays a central role in kinetochore protein assembly, mitotic cell cycle progression and chromosome segregation.
  • Cellular localization

    Cytoplasmic and Nuclear. Note: Nuclear in non confluent cells and cytoplasmic in confluent or dividing cells. Localizes in the kinetochore domain of centromeres.
  • Database links

  • Alternative names

    • bK250D10.2 antibody
    • C22orf18 antibody
    • CENP M antibody
    • Centromere protein M antibody
    • ICEN 39 antibody
    • ICEN39 antibody
    • Interphase centromere complex protein 39 antibody
    • MGC861 antibody
    • OTTHUMP00000028741 antibody
    • PANE 1 antibody
    • PANE1 antibody
    • Proliferation associated nuclear element 1 antibody
    • Proliferation associated nuclear element protein 1 antibody
    see all

Images

  • PFA fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling CENPM using ab243820 at 4 µg/ml (green) in ICC/IF.

  • Anti-CENPM antibody (ab243820) at 0.4 µg/ml + HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

    Predicted band size: 20 kDa

  • Formalin-fixed, paraffin-embedded human lymph node tissue stained for CENPM with ab243820 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for CENPM with ab243820 at a 1/50 dilution in immunohistochemical analysis. Low expression as expected.

References

ab243820 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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