Product nameAnti-CENPT antibody
See all CENPT primary antibodies
DescriptionRabbit polyclonal to CENPT
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- U-251 MG cell lysate; Human plasma; Human liver and Human tonsil tissue lysates; U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|WB||1/100 - 1/250. Predicted molecular weight: 60 kDa.|
FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
Sequence similaritiesBelongs to the CENPT family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
- Information by UniProt
- C16orf56 antibody
- CENP T antibody
- CENP-T antibody
All lanes : Anti-CENPT antibody (ab220280) at 1/100 dilution
Lane 1 : RT-4 cell line lysate
Lane 2 : U-251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 60 kDa
Immunofluorescent analysis of PFA fixed, Triton X-100 permeabilized U-2 OS cells labeling CENPT with ab220280 at 4 μg/mL (green).
ab14404 staining CENPT in the HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 21°C. ab150081 (1/200) used as the secondary antibody.
ab220280 has not yet been referenced specifically in any publications.