Overview

  • Product name

  • Description

    Rabbit polyclonal to CENPT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 480 - 520 (LEMVEKCLDK YFQHLCDDLE VFAAHAGRKT VKPEDLELLM) of Human CENPT (NP_079358.3)

  • Positive control

    • Whole cell lysate from HeLa cells or 293T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab86595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 60 kDa.
IP Use at 10 µg/mg of lysate.

Target

  • Function

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
  • Sequence similarities

    Belongs to the CENPT family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
  • Information by UniProt
  • Database links

  • Alternative names

    • C16orf56 antibody
    • CENP T antibody
    • CENP-T antibody
    • Cenpt antibody
    • CENPT_HUMAN antibody
    • Centromere protein T antibody
    • Chromosome 16 open reading frame 56 antibody
    • ICEN22 antibody
    • Interphase centromere complex protein 22 antibody
    see all

Images

  • All lanes : Anti-CENPT antibody (ab86595) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Predicted band size: 60 kDa


    Exposure time: 3 minutes
  • 1mg whole cell lysate from Hela cells was immunoprecipited using 10ug ab86594.
    20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
    Detection: hemiluminescence with an exposure time of 30 seconds.

References

ab86595 has not yet been referenced specifically in any publications.

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