Key features and details
- Rabbit polyclonal to CENPT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CENPT antibody
See all CENPT primary antibodies
DescriptionRabbit polyclonal to CENPT
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
- Whole cell lysate from HeLa cells or 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 60 kDa.|
|IP||Use at 10 µg/mg of lysate.|
FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function.
Sequence similaritiesBelongs to the CENPT family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes exclusively in the kinetochore domain of centromeres.
- Information by UniProt
- C16orf56 antibody
- CENP T antibody
- CENP-T antibody
All lanes : Anti-CENPT antibody (ab86595) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size: 60 kDa
Exposure time: 3 minutes
ab86595 has not yet been referenced specifically in any publications.