CENTA, beta-lactamase substrate, Yellow chromogenic beta-lactamase substrate (ab145626)

Overview

  • Product name

    CENTA, beta-lactamase substrate, Yellow chromogenic beta-lactamase substrate
  • Description

    Yellow chromogenic β-lactamase substrate
  • Biological description

    Yellow chromogenic β-lactamase substrate. Hydrolyses readily in the presence of all β-lactamases except for Aeromonas hydrophila metalloenzyme. Suitable for kinetic studies, the detection of the enzymes in crude extracts and chromatographic fractions. Absorbs light at 346 nm (Δε = -2500 M-1 cm-1) or 405 nm (Δε = +6400 M-1 cm-1).
  • Purity

    > 98%
  • CAS Number

    9073-60-3
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    (6R,7R)-3-[(3-Carboxy-4-nitrophenyl)sulfanylmethyl]-8-oxo-7-[(2-thiophen-2-ylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
  • Molecular weight

    535.57
  • Molecular formula

    C21H17N3O8S3
  • PubChem identifier

    133358
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in PBS to 25 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C1C(=C(N2[C@H](S1)[C@@H](C2=O)NC(=O)CC3=CC=CS3)C(=O)O)CSC4=CC(=C(C=C4)[N+](=O)[O-])C(=O)O
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Morin S  et al. Backbone resonance assignments of an artificially engineered TEM-1/PSE-4 Class A ß-lactamase chimera. Biomol NMR Assign 4:127-30 (2010). Read more (PubMed: 20383614) »
  • Bebrone C  et al. CENTA as a chromogenic substrate for studying beta-lactamases. Antimicrob Agents Chemother 45:1868-71 (2001). Read more (PubMed: 11353639) »
  • Jones RN  et al. In vitro evaluation of CENTA, a new beta-lactamase-susceptible chromogenic cephalosporin reagent. J Clin Microbiol 15:954-8 (1982). Read more (PubMed: 7047560) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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